Faculty of Food Science, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences-SGGW, Nowoursynowska Str. 159c, 02-776 Warsaw, Poland.
Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Department of Food Analysis, Rakowiecka Str. 36, 02-532 Warsaw, Poland.
Toxins (Basel). 2019 Mar 30;11(4):192. doi: 10.3390/toxins11040192.
Mycotoxins are harmful contaminants of food and feed worldwide. Feed additives with the abilities to trap mycotoxins are considered substances which regulate toxin transfer from feed to tissue, reducing its absorption in animal digestive tract. Market analysis emphasizes growing interest of feed producers in mycotoxins binders obtained from yeast biomass. The aim of the study was to prescreen cell walls (CW) and (1,3)/(1,6)-glucan (-G) preparations isolated from ATCC 9950 cultivated on waste potato juice water with glycerol as adsorbents for aflatoxin B1 (AFB1), zearalenone (ZEN), ochratoxin A (OTA), deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T-2) and fumonisin B1 (FB1). The adsorption was studied in single concentration tests at pH 3.0 and 6.0 in the presence of 1% of the adsorbent and 500 ng/mL of individual toxin. Evaluated CW and -G preparations had the potential to bind ZEN, OTA and AFB1 rather than DON, NIV, T-2 toxin and FB1. The highest percentage of adsorption (about 83%), adsorption capacity (approx. 41 µg/ g preparation) and distribution coefficient (458.7mL/g) was found for zearalenone when CW preparation was used under acidic conditions. Higher protein content in CW and smaller particles sizes of the formulation could influence more efficient binding of ZEN, OTA, DON and T-2 toxin at appropriate pH compared to purified -G. Obtained results show the possibility to transform the waste potato juice water into valuable ATCC 9950 preparation with mycotoxins adsorption properties. Further research is important to improve the binding capacity of studied preparations by increasing the active surface of adsorption.
真菌毒素是全球范围内食品和饲料的有害污染物。具有捕获真菌毒素能力的饲料添加剂被认为是调节毒素从饲料向组织转移的物质，从而减少其在动物消化道中的吸收。市场分析强调了饲料生产商对从酵母生物量中获得的真菌毒素结合物越来越感兴趣。本研究的目的是从 ATCC 9950 细胞中预先筛选细胞壁 (CW) 和 (1,3)/(1,6)-葡聚糖 (-G) 制剂，该细胞在含有甘油的废土豆汁水中培养，用作黄曲霉毒素 B1 (AFB1)、玉米赤霉烯酮 (ZEN)、赭曲霉毒素 A (OTA)、脱氧雪腐镰刀菌烯醇 (DON)、雪腐镰刀菌烯醇 (NIV)、T-2 毒素 (T-2) 和伏马菌素 B1 (FB1) 的吸附剂。在 pH 值为 3.0 和 6.0 的单一浓度试验中，在 1%的吸附剂和 500ng/mL 的单个毒素存在下，研究了吸附作用。评估的 CW 和 -G 制剂具有结合 ZEN、OTA 和 AFB1 的潜力，而不是 DON、NIV、T-2 毒素和 FB1。在酸性条件下使用 CW 制剂时，发现 ZEN 的吸附率（约 83%）、吸附容量（约 41µg/g 制剂）和分配系数（458.7mL/g）最高。在 CW 中，较高的蛋白质含量和较小的制剂颗粒大小可能会影响在适当 pH 值下更有效地结合 ZEN、OTA、DON 和 T-2 毒素，与纯化的 -G 相比。研究结果表明，将废土豆汁转化为具有真菌毒素吸附性能的有价值的 ATCC 9950 制剂是可行的。通过增加吸附活性表面，进一步研究对于提高研究制剂的结合能力很重要。
