Daetwyler Stephan, Huisken Jan
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Biol Bull. 2016 Aug;231(1):14-25. doi: 10.1086/689588.
In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of very fast biological processes, large samples, and high-throughput applications in areas such as neuroscience, plant biology, and developmental biology. This review explains why light sheet microscopes are much faster and gentler than other established fluorescence microscopy techniques. New volumetric imaging schemes and highlights of selected biological applications are also discussed.
在光片显微镜技术中，通过用一片光进行选择性荧光激发来进行光学切片，并结合快速全帧采集。这种照明方案可将光漂白和光毒性降至最低。辅以远程聚焦和多视图采集，光片显微镜技术是用于采集非常快速的生物过程、大样本以及神经科学、植物生物学和发育生物学等领域高通量应用的首选方法。本综述解释了为什么光片显微镜比其他已确立的荧光显微镜技术更快且更温和。还讨论了新的体积成像方案以及选定生物应用的亮点。
