Miller Virginia M, Garovic Vesna D, Bailey Kent R, Lahr Brian D, Mielke Michelle M, White Wendy M, Jayachandran Muthuvel
Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.
General Internal Medicine, Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN 55905, USA.
Atherosclerosis. 2016 Oct;253:150-155. doi: 10.1016/j.atherosclerosis.2016.09.006. Epub 2016 Sep 9.
Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women.
Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age- and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n = 39) or normotensive pregnancy (NP, n = 40). CAC was determined by computed tomography.
CAC scores ranged from 0 to 47 and 0-602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (ρ = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference.
In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined.
有子痫前期病史会增加未来冠状动脉钙化（CAC）的风险。本研究评估了血源性细胞衍生微泡（MV）与中年女性CAC之间的关联。
通过数字流式细胞术检测了年龄和胎次匹配、无心血管事件病史但有子痫前期病史（PE，n = 39）或血压正常妊娠史（NP，n = 40）的女性血液中12种预先选定的抗原特异性MV。通过计算机断层扫描确定CAC。
NP组和PE组的CAC评分分别为0至47和0至602阿加斯顿单位。有CAC的PE女性的腰围和胰岛素抵抗最大。NP组和PE组中组织因子或干细胞/祖细胞抗原（CD117）呈阳性的MV有所不同。在单变量分析中，PE组中组织因子、ICAM-1、干细胞和脂肪细胞（P16-set）抗原呈阳性的与CAC相关。主成分（PC）分析将MV变量减少到三个独立维度。PC1在PE组中与CAC评分有适度相关性（ρ = 0.31，p = 0.06），并且在包括所有3个PC变量的合并组的多变量模型中，在调整妊娠状态后与CAC相关（p = 0.03）。在校正体重指数或腰围后，这种关联消失。
在有PE病史且代谢风险较高的女性中，一组特定抗原阳性的MV与CAC相关。这些MV可能反映了与CAC相关的细胞过程。它们对CAC的诊断潜力仍有待确定。
