Mizobe Yamato, Oya Naoto, Iwakiri Reiko, Yoshida Naomi, Sato Yumi, Onoue Nanase, Miyoshi Kazuchika, Tokunaga Makoto, Ezono Yuji
Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan.
The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
J Assist Reprod Genet. 2016 Dec;33(12):1685-1690. doi: 10.1007/s10815-016-0811-4. Epub 2016 Sep 17.
In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.
Oocytes were divided into three groups-normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.
The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7-67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).
The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.
在对卵膜脆弱的卵母细胞（脆弱卵母细胞）进行胞浆内单精子注射（ICSI）时，注射过程中可能会发生破裂。在本研究中，我们通过ICSI从卵膜脆弱和正常的卵母细胞（正常卵母细胞）中产生胚胎，并比较它们的体外受精和发育能力。我们还研究了脆弱卵母细胞来源的胚胎在囊胚移植后是否能够着床，以确定脆弱卵母细胞是否应用于辅助生殖技术治疗。
将卵母细胞分为三组：不含脆弱卵母细胞周期的正常卵母细胞（A组）、至少含有一个脆弱卵母细胞周期的正常卵母细胞（B组）和脆弱卵母细胞（C组），比较ICSI后它们的受精能力以及所得胚胎的发育能力。
C组的受精率（65.3%）显著低于A组（84.6%）和B组（86.9%）（P<0.01），C组的退化率（24.2%）显著高于A组（0.71%）和B组（0.28%）（P<0.01）。然而，不同组胚胎的囊胚形成率（59.7 - 67.5%）没有显著差异。此外，C组囊胚移植后的妊娠率（50.0%）与A组（35.6%）和B组（45.8%）没有显著差异。
脆弱卵母细胞ICSI后的受精能力低于正常卵母细胞，但所得胚胎与正常卵母细胞来源的胚胎具有相同的发育能力。
