Matczuk Jan, Zalewska Anna, Łukaszuk Bartłomiej, Garbowska Marta, Chabowski Adrian, Żendzian-Piotrowska Małgorzata, Kurek Krzysztof
County Veterinary Inspection, Poland.
Medical University of Bialystok, Department of Conservative Dentistry, Poland.
Prostaglandins Other Lipid Mediat. 2016 Nov;126:9-15. doi: 10.1016/j.prostaglandins.2016.08.003. Epub 2016 Sep 14.
Diabetes is one of the most common metabolic diseases. Moreover, previous studies indicate that diabetes may cause changes in the salivary glands phenotype and in the composition of saliva itself. Therefore, the main objective of this study was to determine the effects of streptozotocin induced diabetes on lipid profile of the rat salivary glands.
Male Wistar rats were divided into two groups: control and STZ-induced diabetes. At the end of the experiment all animals were sacrificed and samples of the parotid and submandibular salivary glands were excised. Major lipid fractions concentrations were determined by means of chromatography (TLC and GC).
We observed a significant increase (∼3.5 fold) in the level of triacylglycerol in both the parotid and submandibular salivary glands of diabetic rats. The abovementioned changes were accompanied by significant, although less dramatic (i.e. from -60% to -90%), decrements in the levels of other lipid classes (phospholipids, free fatty acids and diacylglycerol).
In our study we have showed, presumably for the first time, that streptozotocin induced diabetes causes decrement in PH content with subsequent atrophy and malfunction in both parotid and submandibular salivary glands. Another novel finding of our research is that diabetic rats were characterized by an increased TG accumulation in both parotid and submandibular salivary glands. The later one could be a clinical manifestation of diabetes.
糖尿病是最常见的代谢疾病之一。此外，先前的研究表明，糖尿病可能会导致唾液腺表型以及唾液本身成分的变化。因此，本研究的主要目的是确定链脲佐菌素诱导的糖尿病对大鼠唾液腺脂质谱的影响。
将雄性Wistar大鼠分为两组：对照组和链脲佐菌素诱导的糖尿病组。实验结束时，处死所有动物，切除腮腺和颌下唾液腺样本。通过色谱法（薄层色谱法和气相色谱法）测定主要脂质成分的浓度。
我们观察到糖尿病大鼠的腮腺和颌下唾液腺中三酰甘油水平显著升高（约3.5倍）。上述变化伴随着其他脂质类（磷脂、游离脂肪酸和二酰甘油）水平的显著下降，尽管下降幅度较小（即从-60%到-90%）。
在我们的研究中，我们大概首次表明，链脲佐菌素诱导的糖尿病会导致腮腺和颌下唾液腺的磷脂含量下降，随后出现萎缩和功能障碍。我们研究的另一个新发现是，糖尿病大鼠的腮腺和颌下唾液腺中三酰甘油积累增加。后者可能是糖尿病的一种临床表现。
