Koyama S, Rennard S I, Shoji S, Romberger D, Linder J, Ertl R, Robbins R A
Research Service, Omaha Veterans Administration Medical Center, Nebraska 68105.
Am J Physiol. 1989 Aug;257(2 Pt 1):L130-6. doi: 10.1152/ajplung.1989.257.2.L130.
Lung macrophages are monocyte-derived cells that play a central and essential part in local immunity. Because the bronchial epithelial cells that line the airway can modulate their local cellular environment by releasing chemotactic factors for neutrophils, lymphocytes, and fibroblasts, we postulated that the bronchial epithelial cells might release chemotactic activity for monocytes. To test this hypothesis, bovine bronchial epithelial cells were isolated and cultured. The supernatant fluids were collected at 12, 24, 36, 48, 72, 96, and 120 h and evaluated for monocyte chemotactic activity, using a blind-well chamber technique. The supernatant fluids possessed significantly greater chemotactic activity than medium alone, with optimal migration contained in supernatant fluids harvested at 72 h (5.8 +/- 2.3 vs. 39.8 +/- 2.8 cells/high-power field, P less than 0.001). Partial characterization of the released monocyte chemotactic activity revealed that the cells released a low-molecular-weight lipid-soluble chemotactic factor after 24 h in culture, but in contrast, a high-molecular-weight protein chemokinetic factor was released after 72 h in culture. These findings suggest that bronchial epithelial cells may release chemotactic activity for, and thus may modulate the recruitment of, monocytes into bronchial passages.
肺巨噬细胞是源自单核细胞的细胞，在局部免疫中发挥着核心且至关重要的作用。由于气道内衬的支气管上皮细胞可通过释放针对中性粒细胞、淋巴细胞和成纤维细胞的趋化因子来调节其局部细胞环境，我们推测支气管上皮细胞可能会释放针对单核细胞的趋化活性。为验证这一假设，分离并培养了牛支气管上皮细胞。在12、24、36、48、72、96和120小时收集上清液，并使用盲孔室技术评估单核细胞趋化活性。上清液的趋化活性明显高于单独的培养基，72小时收集的上清液中趋化活性最佳（5.8±2.3对39.8±2.8个细胞/高倍视野，P<0.001）。对释放的单核细胞趋化活性的部分特性分析表明，细胞在培养24小时后释放出一种低分子量脂溶性趋化因子，但相反，培养72小时后释放出一种高分子量蛋白质趋化动力学因子。这些发现表明，支气管上皮细胞可能会释放针对单核细胞的趋化活性，从而可能调节单核细胞向支气管通道的募集。
