Robbins R A, Shoji S, Linder J, Gossman G L, Allington L A, Klassen L W, Rennard S I
Research Service, Omaha Veterans Administration Medical Center, Omaha, Nebraska 68105.
Am J Physiol. 1989 Aug;257(2 Pt 1):L109-15. doi: 10.1152/ajplung.1989.257.2.L109.
Lymphocytes can frequently be observed in association with bronchial tissues. One mechanism that might account for this association is that bronchial epithelial cells might release chemotactic factors for lymphocytes. To test this hypothesis, bovine bronchial epithelial cells were cultured in serum-free media, and the supernatant fluids were harvested and evaluated for lymphocyte chemotactic activity using a blind-well chamber technique. Media alone attracted few lymphocytes (12 +/- 2 cells/high power field), but in contrast, there was a significant increase in the number of cells attracted by supernatant fluids obtained from bronchial epithelial cell cultures (40 +/- 6 cells/high power field, P = 0.002). The activity was dose dependent and was demonstrated to be chemotactic activity by checkerboard analysis. Partial characterization of the activity revealed it was not extractable into ethyl acetate but was partially inactivated by trypsin and heat (100 degrees C, 15 min). The responding cells were predominantly T-helper lymphocytes as shown by monoclonal antibody staining, with a smaller proportion being B-lymphocytes. Molecular sieve column chromatography revealed multiple peaks of lymphocyte chemotactic activity, with three of the peaks preferentially attracting T-helper lymphocytes and one of the peaks preferentially attracting B-lymphocytes. These data demonstrate that bronchial epithelial cells can release chemotactic factors for lymphocytes and suggest that bronchial epithelial cells may modulate their local population of immune effector cells.
淋巴细胞常可在支气管组织中观察到。可能解释这种关联的一种机制是支气管上皮细胞可能释放淋巴细胞趋化因子。为验证这一假设，将牛支气管上皮细胞在无血清培养基中培养，收集上清液，并使用盲孔室技术评估其淋巴细胞趋化活性。单独的培养基吸引的淋巴细胞很少（每高倍视野12±2个细胞），但相比之下，从支气管上皮细胞培养物中获得的上清液吸引的细胞数量显著增加（每高倍视野40±6个细胞，P = 0.002）。该活性呈剂量依赖性，通过棋盘分析证明为趋化活性。对该活性的部分特性分析表明，它不能被乙酸乙酯提取，但可被胰蛋白酶和加热（100℃，15分钟）部分灭活。单克隆抗体染色显示，反应细胞主要是辅助性T淋巴细胞，较小比例为B淋巴细胞。分子筛柱层析显示淋巴细胞趋化活性有多个峰，其中三个峰优先吸引辅助性T淋巴细胞，一个峰优先吸引B淋巴细胞。这些数据表明支气管上皮细胞可释放淋巴细胞趋化因子，并提示支气管上皮细胞可能调节其局部免疫效应细胞群体。
