Abreu Ana Cristina, Saavedra Maria José, Simões Lúcia C, Simões Manuel
a LEPABE, Department of Chemical Engineering, Faculty of Engineering , University of Porto , Porto , Portugal.
b CECAV, Veterinary and Animal Science Research Center, Quality and Food Safety of Animal Products Group , University of Trás-os-Montes and Alto Douro , Vila Real , Portugal.
Biofouling. 2016 Oct;32(9):1103-14. doi: 10.1080/08927014.2016.1232402.
Combinations of selected phytochemicals (reserpine, pyrrolidine, quinine, morin and quercetin) with antibiotics (ciprofloxacin, tetracycline and erythromycin) were tested on the prevention and control of Staphylococcus aureus biofilms. The phytochemicals were also studied for their ability to avoid antibiotic adaptation and to inhibit antibiotic efflux pumps. Morin, pyrrolidine and quercetin at subinhibitory concentrations had significant effects in biofilm prevention and/or control when applied alone and combined with antibiotics. Synergism between antibiotics and phytochemicals was found especially against biofilms of NorA overexpressing strain S. aureus SA1199B. This strain when growing with subinhibitory concentrations of ciprofloxacin developed increased tolerance to this antibiotic. However, this was successfully reversed by quinine and morin. In addition, reserpine and quercetin showed significant efflux pump inhibition. The overall results demonstrate the role of phytochemicals in co-therapies to promote more efficient treatments and decrease antimicrobial resistance to antibiotics, with substantial effects against S. aureus in both planktonic and biofilm states.
研究了选定的植物化学物质（利血平、吡咯烷、奎宁、桑色素和槲皮素）与抗生素（环丙沙星、四环素和红霉素）的组合对金黄色葡萄球菌生物膜的预防和控制作用。还研究了这些植物化学物质避免抗生素适应性和抑制抗生素外排泵的能力。亚抑制浓度的桑色素、吡咯烷和槲皮素单独使用以及与抗生素联合使用时，对生物膜的预防和/或控制具有显著效果。发现抗生素与植物化学物质之间存在协同作用，尤其是对过表达NorA的金黄色葡萄球菌SA1199B菌株的生物膜。该菌株在亚抑制浓度的环丙沙星中生长时，对这种抗生素的耐受性增强。然而，奎宁和桑色素成功逆转了这种情况。此外，利血平和槲皮素显示出显著的外排泵抑制作用。总体结果表明，植物化学物质在联合治疗中发挥作用，以促进更有效的治疗并降低对抗生素的耐药性，对浮游和生物膜状态的金黄色葡萄球菌均有显著效果。
