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具有抗生物膜和抗菌作用的肉桂醛和庆大霉素负载双层角膜纳米纤维贴片的设计

Design of Cinnamaldehyde- and Gentamicin-Loaded Double-Layer Corneal Nanofiber Patches with Antibiofilm and Antimicrobial Effects.

作者信息

Cesur Sumeyye, Ilhan Elif, Tut Tufan Arslan, Kaya Elif, Dalbayrak Basak, Bosgelmez-Tinaz Gulgun, Arısan Elif Damla, Gunduz Oguzhan, Kijeńska-Gawrońska Ewa

机构信息

Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey.

Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul 34722, Turkey.

出版信息

ACS Omega. 2023 Jul 26;8(31):28109-28121. doi: 10.1021/acsomega.3c00914. eCollection 2023 Aug 8.

DOI:10.1021/acsomega.3c00914
PMID:37576652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10413367/
Abstract

In this study, two-layer poly(vinyl alcohol)/gelatin (PVA/GEL) nanofiber patches containing cinnamaldehyde (CA) in the first layer and gentamicin (GEN) in the second layer were produced by the electrospinning method. The morphology, chemical structures, and thermal temperatures of the produced pure (PVA/GEL), CA-loaded (PVA/GEL/CA), GEN-loaded (PVA/GEL/GEN), and combined drug-loaded (PVA/GEL/CA/GEN) nanofiber patches were determined by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and differential scanning calorimetry, respectively. Their mechanical properties, swelling and degradation behavior, and drug release kinetics were investigated. SEM images showed that both drug-free and drug-loaded nanofiber patches possess smooth and monodisperse structures, and nanofiber size increase occurred as the amount of drug increased. The tensile test results showed that the mechanical strength decreased as the drug was loaded. According to the drug release results, CA release ended at the 96th hour, while GEN release continued until the 264th hour. The antibacterial and antibiofilm activities of PVA/GEL, PVA/GEL/CA, PVA/GEL/GEN, and PVA/GEL/CA/GEN nanofiber patches against and were evaluated. Results showed that PVA/GEL/GEN and PVA/GEL/CA/GEN nanofiber patches have excellent antibacterial and antibiofilm activities. Moreover, all materials were biocompatible, with no cytotoxic effects in the mammalian cell model for 8 days. PVA/GEL/GEN nanofiber patches were the most promising material for a high cell survival ratio, which was confirmed by SEM images. This research aims to develop an alternative method to stop and treat the rapid progression of bacterial keratitis.

摘要

在本研究中，通过静电纺丝法制备了两层聚（乙烯醇）/明胶（PVA/GEL）纳米纤维贴片，第一层含有肉桂醛（CA），第二层含有庆大霉素（GEN）。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱和差示扫描量热法分别测定了制备的纯（PVA/GEL）、载CA（PVA/GEL/CA）、载GEN（PVA/GEL/GEN）和联合载药（PVA/GEL/CA/GEN）纳米纤维贴片的形态、化学结构和热温度。研究了它们的力学性能、溶胀和降解行为以及药物释放动力学。SEM图像显示，无药和载药纳米纤维贴片均具有光滑且单分散的结构，并且随着药物含量的增加，纳米纤维尺寸增大。拉伸试验结果表明，载药后力学强度降低。根据药物释放结果，CA在第96小时释放结束，而GEN释放持续至第264小时。评估了PVA/GEL、PVA/GEL/CA、PVA/GEL/GEN和PVA/GEL/CA/GEN纳米纤维贴片对[具体菌种1]和[具体菌种2]的抗菌和抗生物膜活性。结果表明，PVA/GEL/GEN和PVA/GEL/CA/GEN纳米纤维贴片具有优异的抗菌和抗生物膜活性。此外，所有材料均具有生物相容性，在哺乳动物细胞模型中8天内无细胞毒性作用。SEM图像证实，PVA/GEL/GEN纳米纤维贴片是细胞存活率最高的最有前景的材料。本研究旨在开发一种替代方法来阻止和治疗细菌性角膜炎的快速进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/b928349f0597/ao3c00914_0012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/b928349f0597/ao3c00914_0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/f01b55edf7b3/ao3c00914_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/f0326dc2d849/ao3c00914_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/9d11e33fa39e/ao3c00914_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/3f092815e6e9/ao3c00914_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/cbe183e53b95/ao3c00914_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/f60017e3909d/ao3c00914_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/0eeb8274e5a9/ao3c00914_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/5d19ecff1f55/ao3c00914_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/43dd2d8ba63d/ao3c00914_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f65b/10413367/b928349f0597/ao3c00914_0012.jpg

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