Bernardet J F, Kerouault B
Institut National de la Recherche Agronomique, Laboratoire dhIchtyopathologie, Jouy-en-Josas, France.
Appl Environ Microbiol. 1989 Jul;55(7):1796-800. doi: 10.1128/aem.55.7.1796-1800.1989.
Five strains of gliding bacteria were isolated in France from farmed diseased rainbow trouts reared at low water temperature. The resemblance of these bacteria to the known fish pathogen "Cytophaga psychrophila" led to their comparative study with reference strain NCMB 1947 and with an American isolate. Morphological, physiological, and biochemical characteristics of the seven strains proved to be similar. Comparison of their DNA by the S1 nuclease DNA-DNA hybridization method showed that the seven strains formed a tight genomic species with DNA relatedness above 90%. This is the first identification of this fish pathogen in a European country. The main phenotypic characteristics differentiating this bacterium from other nonpathogenic gliding bacteria of fish origin include a poor gliding movement, yellow compact or weakly rhizoid colonies on solid media, and the presence of flexirubin-type pigments. The inability to metabolize any carbohydrates, the strong proteolytic activity, the absence of growth in more than 0.5% NaCl, and the tolerance to a maximum temperature of 25 degrees C are also useful characteristics of this group of bacteria.
在法国，从低温养殖的患病虹鳟鱼中分离出了5株滑行细菌。这些细菌与已知的鱼类病原体“嗜冷纤维黏细菌”相似，因此对其与参考菌株NCMB 1947以及一株美国分离株进行了比较研究。7株菌株的形态、生理和生化特征被证明是相似的。通过S1核酸酶DNA-DNA杂交方法对它们的DNA进行比较，结果显示这7株菌株形成了一个紧密的基因组种，DNA相关性超过90%。这是在欧洲国家首次鉴定出这种鱼类病原体。将这种细菌与其他鱼类来源的非致病性滑行细菌区分开来的主要表型特征包括滑行运动较弱、在固体培养基上形成黄色致密或弱根状菌落以及存在flexirubin型色素。不能代谢任何碳水化合物、强烈的蛋白水解活性、在超过0.5% NaCl中不生长以及对最高温度25摄氏度的耐受性也是这组细菌的有用特征。
