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青黛中新纯化多糖的提取、表征及生物活性

Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix.

作者信息

Du Jiali, Wang Baolan, Chen Jianhui, Zhang Zhe, Li Shijie, He Lian, Lai Xiaoping, Zhang Danyan, Wang Kanglin

机构信息

School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.

Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu, PR China.

出版信息

Int J Biol Macromol. 2016 Dec;93(Pt A):879-888. doi: 10.1016/j.ijbiomac.2016.09.055. Epub 2016 Sep 16.

DOI:10.1016/j.ijbiomac.2016.09.055
PMID:27645928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7112370/
Abstract

The purpose of this study was to investigate the extraction, characterization and bioactivities of purified water-soluble polysaccharides (BCP) from Baphicacanthis Cusiae Rhizoma et Radix. Based on the response surface methodology, the optimal extraction parameters were obtained as follows: extraction temperature of 60.0°C, extraction time of 35.0min, and ratio of water to raw material of 24.5ml/g. Then, BCP was separated and purified by chromatography of DEAE-52 and Sephadex G-100, and obtained two purified fractions, named as BCP-1 and BCP-2. Their molecular weights were respectively 11.6 and 26.7 KDa with mainly composed of glucose, arabinose and galactose. BCP-2 had higher contents of sulfuric radical and uronic acid than BCP-1. Finally, their antioxidant and anti-inflammatory activities were evaluated. Both of BCP-1 and BCP-2 exhibited strong antioxidant activity in vitro, and the antioxidant of BCP-2 was better. Besides, they showed ideal anti-inflammatory activity in vitro and in vivo.

摘要

本研究旨在探讨马蓝根茎和根中纯化的水溶性多糖（BCP）的提取、表征及生物活性。基于响应面法，得到最佳提取参数如下：提取温度60.0℃、提取时间35.0分钟、料液比24.5ml/g。然后，通过DEAE - 52和Sephadex G - 100柱层析对BCP进行分离纯化，得到两个纯化级分，命名为BCP - 1和BCP - 2。它们的分子量分别为11.6和26.7 kDa，主要由葡萄糖、阿拉伯糖和半乳糖组成。BCP - 2的硫酸根和糖醛酸含量高于BCP - 1。最后，对它们的抗氧化和抗炎活性进行了评价。BCP - 1和BCP - 2在体外均表现出较强的抗氧化活性，且BCP - 2的抗氧化性能更佳。此外，它们在体外和体内均表现出理想的抗炎活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/b4a4d798e1ad/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/001e41832110/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/9d38bd5710f3/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/6fb30fc44763/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/a5d745323965/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/b4a4d798e1ad/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/001e41832110/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/9d38bd5710f3/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/6fb30fc44763/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/a5d745323965/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f6a/7112370/b4a4d798e1ad/gr5_lrg.jpg

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