a Department of Anatomy , Bharath University , Selaiyur, Chennai , Tamil Nadu 600073 , India.
b Department of Anatomy , Velammal Medical College and Hospital , Madurai , Tamil Nadu 625009 , India.
Nutr Neurosci. 2018 Feb;21(2):97-107. doi: 10.1080/1028415X.2016.1222332. Epub 2016 Sep 20.
The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).
本研究旨在探讨灵芝提取物对鱼藤酮诱导的细胞模型的影响。根据 MTT 测定结果，将 SH-SY5Y 神经母细胞瘤细胞分为四组实验（对照组、鱼藤酮（100nM）组、灵芝（5μg/ml）+鱼藤酮（100nM）组和灵芝单独处理组），测量细胞内 ROS、TBARS 水平和抗氧化剂活性。最后，还分析了线粒体跨膜电位（MMP）和凋亡蛋白的表达。灵芝的预处理显著提高了细胞活力，减弱了鱼藤酮诱导的 ROS、MMP 和凋亡。我们的结果表明，这种天然化合物的抗凋亡特性归因于其抗氧化和线粒体保护功能，可保护鱼藤酮诱导的细胞毒性。因此，可以得出结论，灵芝可以进一步开发为治疗帕金森病（PD）的有前途的药物。
