Bauer H, Dimitriadis E A, Snyder R
Joint Graduate Program in Toxicology, Rutgers the State University of New Jersey, Piscataway 08855.
Arch Toxicol. 1989;63(3):209-13. doi: 10.1007/BF00316370.
Rabbits were treated with benzene (586 mg/kg/b.i.d./4 days) after which DNA was isolated from liver and analyzed for adduct formation using the [32P] post-labeling method of Randerath and coworkers (Randerath et al. 1981; Reddy et al. 1984, 1986, 1987). Liver 500 g and 9000 g fractions were analyzed for adducts. There appeared to be several adducts in both the 500 g and 9000 g fractions observed on radioautographs of cellulose-TLC plates. Several adducts were also observed when the 9000 g fraction was studied using HPLC.
兔子经苯（586毫克/千克/每日两次/共4天）处理后，从肝脏中分离出DNA，并使用Randerath及其同事的[32P]后标记法（Randerath等人，1981年；Reddy等人，1984年、1986年、1987年）分析加合物的形成。对肝脏500克和9000克组分进行加合物分析。在纤维素薄层层析板的放射自显影片上观察到，500克和9000克组分中似乎都有几种加合物。当使用高效液相色谱法研究9000克组分时，也观察到了几种加合物。
