接触苯后在尿液中发现苯基鸟嘌呤。
Phenylguanine found in urine after benzene exposure.
作者信息
Norpoth K H, Müller G, Schell C, Jorg E
机构信息
Institute of Hygiene and Occupational Medicine, University Medical Center, Essen, Germany.
出版信息
Environ Health Perspect. 1996 Dec;104 Suppl 6(Suppl 6):1159-63. doi: 10.1289/ehp.961041159.
Abstract
Comparative investigations with synthetic N7-phenylguanine were carried out to clarify whether this compound is eliminated via the urine of rats as a benzene-derived nucleic acid adduct. As sensitive methods for detecting trace amounts of the compound, gas chromatography-mass spectroscopy, high performance liquid chromatography, and two immunoassays (enzyme-linked immunosorbent assay and fluoroimmunoassay) with appropriate monoclonal antibodies were used. The results indicate the excretion of several benzene-related guanine adducts slightly different from N7-phenylguanine that may possibly be hydroxylated. These adducts differ also from (O6-, N2- and C8-phenylguanine, respectively.
摘要
开展了与合成N7-苯基鸟嘌呤的对比研究，以阐明该化合物是否作为苯衍生的核酸加合物通过大鼠尿液排出。作为检测痕量该化合物的灵敏方法，使用了气相色谱-质谱联用、高效液相色谱以及两种带有合适单克隆抗体的免疫测定法（酶联免疫吸附测定法和荧光免疫测定法）。结果表明，有几种与苯相关的鸟嘌呤加合物排出，它们与N7-苯基鸟嘌呤略有不同，可能发生了羟基化。这些加合物也分别与（O6-、N2-和C8-苯基鸟嘌呤不同。
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