Batista Philip D, Janes Jasmine K, Boone Celia K, Murray Brent W, Sperling Felix A H
Department of Biological Sciences University of Alberta Edmonton Alberta T6G2E9 Canada.
Department of Biological Sciences University of Alberta Edmonton Alberta T6G2E9 Canada; School of Environmental and Rural Science University of New England Armidale New South Wales 2351 Australia.
Ecol Evol. 2016 Aug 8;6(17):6292-300. doi: 10.1002/ece3.2367. eCollection 2016 Sep.
Assessments of population genetic structure and demographic history have traditionally been based on neutral markers while explicitly excluding adaptive markers. In this study, we compared the utility of putatively adaptive and neutral single-nucleotide polymorphisms (SNPs) for inferring mountain pine beetle population structure across its geographic range. Both adaptive and neutral SNPs, and their combination, allowed range-wide structure to be distinguished and delimited a population that has recently undergone range expansion across northern British Columbia and Alberta. Using an equal number of both adaptive and neutral SNPs revealed that adaptive SNPs resulted in a stronger correlation between sampled populations and inferred clustering. Our results suggest that adaptive SNPs should not be excluded prior to analysis from neutral SNPs as a combination of both marker sets resulted in better resolution of genetic differentiation between populations than either marker set alone. These results demonstrate the utility of adaptive loci for resolving population genetic structure in a nonmodel organism.
对种群遗传结构和种群历史的评估传统上基于中性标记，同时明确排除适应性标记。在本研究中，我们比较了推定的适应性单核苷酸多态性（SNP）和中性SNP在推断山地松甲虫地理分布范围内种群结构方面的效用。适应性SNP和中性SNP及其组合，都能够区分全范围的结构，并划定了一个最近在不列颠哥伦比亚省北部和艾伯塔省经历范围扩张的种群。使用数量相等的适应性SNP和中性SNP表明，适应性SNP导致采样种群与推断聚类之间的相关性更强。我们的结果表明，在分析前不应将适应性SNP从中性SNP中排除，因为两种标记集的组合比单独使用任何一种标记集都能更好地解析种群间的遗传分化。这些结果证明了适应性位点在解析非模式生物种群遗传结构方面的效用。
