Goffredo Martina, Mass Kendra, Parks Elizabeth J, Wagner David A, McClure Emily Ann, Graf Joerg, Savoye Mary, Pierpont Bridget, Cline Gary, Santoro Nicola
Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
J Clin Endocrinol Metab. 2016 Nov;101(11):4367-4376. doi: 10.1210/jc.2016-1797. Epub 2016 Sep 20.
We aimed at determining the relationship of the gut microbiota and short chain fatty acids with obesity and fat partitioning and at testing potential differences in the ability of gut microbiota to ferment equal amounts of carbohydrates (CHO) between lean and obese youth.
We analyzed the gut microbiota of 84 youth in whom body fat distribution was measured by fast-magnetic resonance imaging, de novo lipogenesis (DNL) quantitated using deuterated water, and the capability of gut flora to ferment CHO was assessed by C-fructose treatment in vitro.
A significant association was found between the Firmicutes to Bacteroidetes ratio, and the abundance of Bacteroidetes and Actinobacteria with body mass index, visceral and SC fat (all P < .05). Plasma acetate, propionate, and butyrate were associated with body mass index and visceral and SC fat (all P < .05) and with hepatic DNL (P = .01, P = .09, P = .04, respectively). Moreover, the rate of CHO fermentation from the gut flora was higher in obese than in lean subjects (P = .018).
These data demonstrate that obese youth show a different gut flora composition than lean and that short chain fatty acids are associated with body fat partitioning and DNL. Also, the gut microbiota of obese youth have a higher capability than the gut flora of lean to oxidize CHO.
我们旨在确定肠道微生物群和短链脂肪酸与肥胖及脂肪分布的关系，并测试瘦素和肥胖青少年肠道微生物群发酵等量碳水化合物（CHO）能力的潜在差异。
我们分析了84名青少年的肠道微生物群，通过快速磁共振成像测量其体脂分布，使用氘水定量从头脂肪生成（DNL），并通过体外C-果糖处理评估肠道菌群发酵CHO的能力。
发现厚壁菌门与拟杆菌门的比例、拟杆菌门和放线菌的丰度与体重指数、内脏脂肪和皮下脂肪之间存在显著关联（所有P < 0.05）。血浆乙酸盐、丙酸盐和丁酸盐与体重指数、内脏脂肪和皮下脂肪相关（所有P < 0.05），并与肝脏DNL相关（分别为P = 0.01、P = 0.09、P = 0.04）。此外，肥胖受试者肠道菌群的CHO发酵率高于瘦素受试者（P = 0.018）。
这些数据表明，肥胖青少年的肠道菌群组成与瘦素不同，短链脂肪酸与体脂分布和DNL相关。此外，肥胖青少年的肠道微生物群氧化CHO的能力高于瘦素的肠道菌群。
