Robledo-Márquez Karina, Gutiérrez-Escobedo Guadalupe, Yáñez-Carrillo Patricia, Vidal-Aguiar Yamile, Briones-Martín-Del-Campo Marcela, Orta-Zavalza Emmanuel, De Las Peñas Alejandro, Castaño Irene
División de Biología Molecular, IPICYT-Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San Jose no. 2055, Lomas 4a Seccion, San Luis Potosí, 78216, México.
División de Biología Molecular, IPICYT-Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San Jose no. 2055, Lomas 4a Seccion, San Luis Potosí, 78216, México
FEMS Yeast Res. 2016 Nov;16(7). doi: 10.1093/femsyr/fow082. Epub 2016 Sep 19.
The fungal pathogen Candida glabrata is a haploid asexual yeast. Candida glabrata contains orthologs of the genes that control mating and cell-type identity in other fungi, which encode putative transcription factors localized in the MAT locus in Saccharomyces cerevisiae or MTL in other fungi. Candida glabrata contains three copies of the CgMTL locus but only CgMTL1 correctly expresses the information encoded in it. CgMTL1 can encode the Cg A1: gene ( A: information), or the Cgalpha1 and Cgalpha2 genes (alpha information). CgMTL2 contains an identical copy of the Cg A1: gene. CgMTL3 contains an identical copy of the Cgalpha1 gene but a longer variant of the Cgalpha2 gene that we termed Cgalpha3. In S. cerevisiae diploid cells, that express Sc A: and Scalpha information, Sc A1: and Scalpha2 proteins form a heterodimer, which represses genes expressed only in haploid cells and some genes involved in stress response. We constructed C. glabrata strains that simultaneously express Cg A1: and Cgalpha2 or Cg A1: and Cgalpha3 genes. We did not find any phenotype in these strains when grown under a large variety of stress and nutritional conditions. However, we detected an interaction between Cg A1: and Cgalpha2 but not between Cg A1: and Cgalpha3 by Bimolecular Fluorescence Complementation and co-immunoprecipitation assays.
真菌病原体光滑念珠菌是一种单倍体无性酵母。光滑念珠菌含有其他真菌中控制交配和细胞类型身份的基因的直系同源物，这些基因编码位于酿酒酵母MAT位点或其他真菌MTL中的假定转录因子。光滑念珠菌含有三个CgMTL位点拷贝，但只有CgMTL1能正确表达其中编码的信息。CgMTL1可以编码Cg A1:基因（A:信息），或Cgalpha1和Cgalpha2基因（α信息）。CgMTL2含有Cg A1:基因的相同拷贝。CgMTL3含有Cgalpha1基因的相同拷贝，但Cgalpha2基因的一个较长变体，我们将其称为Cgalpha3。在表达Sc A:和Scα信息的酿酒酵母二倍体细胞中，Sc A1:和Scα2蛋白形成异二聚体，它会抑制仅在单倍体细胞中表达的基因以及一些参与应激反应的基因。我们构建了同时表达Cg A1:和Cgalpha2或Cg A1:和Cgalpha3基因的光滑念珠菌菌株。在多种应激和营养条件下培养时，我们在这些菌株中未发现任何表型。然而，通过双分子荧光互补和免疫共沉淀分析，我们检测到Cg A1:和Cgalpha2之间存在相互作用，但Cg A1:和Cgalpha3之间没有相互作用。
