Sennerstam R, Kato H, Auer G U
Department of Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.
Anal Quant Cytol Histol. 1989 Aug;11(4):255-60.
Tumor progression was analyzed in vivo. The bronchial epithelium in five beagle dogs was weekly treated by 20-methylcholanthrene (20-MC). Bronchial cells were harvested before each application of the drug. The cytologic criteria used in the diagnostic procedure were based on a grading developed for Papanicolaou-stained preparations of human squamous bronchial epithelium. The cells were then destained and restained by Feulgen-naphthol yellow S technique. An increased variation in the protein/DNA ratio was an early event in tumorigenesis; it occurred even before aneuploid cells appeared in mild dysplasia, as compared with the control cells. A large increase in the coefficient of variation (CV) in the protein/DNA ratio in mild dysplasia vis-a-vis the control cells was positively correlated to the degree of aneuploidy occurring later in tumorigenesis. These results were compared with the findings in breast cancer cells from patients with near-diploid, aneuploid and near-tetraploid tumors. The CV in the protein/DNA ratio was significantly higher in the aneuploid tumors, indicating an increased dissociation between cell growth and DNA synthesis.
在体内分析肿瘤进展情况。对五只比格犬的支气管上皮每周用20-甲基胆蒽（20-MC）进行处理。在每次用药前采集支气管细胞。诊断程序中使用的细胞学标准基于为人类鳞状支气管上皮巴氏染色制剂制定的分级标准。然后用福尔根-萘酚黄S技术对细胞进行脱色和重新染色。蛋白质/DNA比值的变异增加是肿瘤发生过程中的早期事件；与对照细胞相比，甚至在轻度发育异常中出现非整倍体细胞之前就已发生。轻度发育异常中蛋白质/DNA比值的变异系数（CV）相对于对照细胞大幅增加，与肿瘤发生后期出现的非整倍体程度呈正相关。将这些结果与来自近二倍体、非整倍体和近四倍体肿瘤患者的乳腺癌细胞的研究结果进行比较。非整倍体肿瘤中蛋白质/DNA比值的CV显著更高，表明细胞生长与DNA合成之间的解离增加。
