采用多重聚合酶链反应对鹿源产志贺毒素大肠杆菌分离株进行基因分型研究。
Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction.
作者信息
Mishra Raghavendra Prasad, Jain Udit, Singh Rakesh Kumar
机构信息
Department of Veterinary Public Health, Veterinary University, UP Pandit Deen Dayal Upadhyaya Veterinary Science University, Mathura, Uttar Pradesh, India.
Veterinary Officer, Kanpur Zoo, Kanpur, Uttar Pradesh, India.
出版信息
Vet World. 2016 Aug;9(8):919-21. doi: 10.14202/vetworld.2016.919-921. Epub 2016 Aug 30.
AIM
This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance.
MATERIALS AND METHODS
A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR).
RESULTS
All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1 , stx2 , eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations.
CONCLUSION
Genes such as stx1 , stx2 , eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health.
目的
鉴于其对公共卫生的重要性，本研究旨在对鹿粪便样本中的产志贺毒素大肠杆菌（VTEC）基因型进行研究。
材料与方法
从马图拉地区和坎普尔动物园采集了总共160份鹿粪便样本，并通过聚合酶链反应（PCR）对VTEC基因进行筛查。
结果
所有粪便样本的大肠杆菌检测均呈阳性。通过PCR对所有大肠杆菌分离株进行筛查，以检测毒力基因stx1、stx2、eaeA和hlyA。其中，15株分离株被发现为VTEC阳性，具有不同组合的一个或多个基因。
结论
stx1、stx2、eaeA和hlyA等基因在鹿粪便中的VTEC分离株中普遍存在。具有毒力基因的VTEC分离株的存在可能对公共卫生构成威胁。
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