Patología Infecciosa y Epidemiología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, 10071 Cáceres, Spain.
Vet Microbiol. 2010 Jul 14;143(2-4):420-3. doi: 10.1016/j.vetmic.2009.11.016. Epub 2009 Dec 11.
The aim of this work was to determine the prevalence and characteristics of Escherichia coli O157:H7 and non-O157 Shiga toxin-producing E. coli (STEC) in free-ranging wild boars killed during the hunting season in southwest Spain. Faecal samples from 212 wild boars (Sus scrofa) were collected and examined for STEC. Characterisation of isolates was performed by PCR, serotyping, phage typing, and pulsed-field gel electrophoresis (PFGE). E. coli O157:H7 and non-O157 STEC were isolated from 7 (3.3%) and 11 (5.2%) animals, respectively, and the resulting 19 isolates were characterised. The PCR procedure indicated that 4 isolates carried the stx(1) gene, 12 carried the stx(2) gene, and 1 contained both of these genes. The ehxA, eae, and saa genes were detected in 13, 8, and 1 of the isolates, respectively. The eae-positive isolates comprised the types eae-gamma 1 and eae-zeta. The isolates belonged to 11 O:H serotypes, including 4 new serotypes not previously reported within STEC strains, and the majority of them were from serotypes previously associated with human infection. E. coli O157:H7 isolates belonged to phage types associated with severe human illness: PT14, PT34, and PT54. Indistinguishable PFGE types were found in E. coli O157:H7 isolates recovered from a wild boar and from a human patient with diarrhoea living in the same geographic area.
本研究旨在确定西班牙西南部狩猎季节中被捕杀的野猪中流行的大肠杆菌 O157:H7 和非 O157 产志贺毒素大肠杆菌（STEC）的流行率和特征。采集了 212 头野猪（Sus scrofa）的粪便样本，并对其进行了 STEC 检测。通过 PCR、血清型分型、噬菌体分型和脉冲场凝胶电泳（PFGE）对分离株进行了特征分析。从 7 头（3.3%）和 11 头（5.2%）动物中分别分离出大肠杆菌 O157:H7 和非 O157 STEC，共分离出 19 株。PCR 程序表明，4 株分离株携带 stx(1)基因，12 株携带 stx(2)基因，1 株同时携带这两种基因。ehxA、eae 和 saa 基因分别在 13、8 和 1 株分离株中被检测到。eae 阳性分离株包括 eae-gamma 1 和 eae-zeta 两种类型。分离株属于 11 种 O:H 血清型，包括 4 种以前未在 STEC 菌株中报道过的新血清型，其中大多数与以前与人类感染相关的血清型有关。大肠杆菌 O157:H7 分离株属于与严重人类疾病相关的噬菌体类型：PT14、PT34 和 PT54。从同一地理区域的一头野猪和一位患有腹泻的人类患者中分离出的大肠杆菌 O157:H7 分离株具有相同的 PFGE 类型。
