Dosi Tanvi, Gupta Dhaman, Hazari Alka, Rajput Rajan, Chauhan Prabhav, Rajapuri Anushri S
Department of Oral Medicine and Radiology, Jodhpur Dental College General Hospital, Jodhpur, India.
Department of Public Health Dentistry, Vyas Dental College and Hospital, Jodhpur, Rajasthan, India.
J Int Soc Prev Community Dent. 2016 Aug;6(Suppl 2):S143-7. doi: 10.4103/2231-0762.189745.
To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation.
Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i.e., 10 patients with habit control and 10 patients with OPLs. Statistical analysis was performed by the Statistical Package for the Social Sciences version 21.0 Unpaired t-test was performed to determine the micronucleated cell (MNC) and micronuclei (MN) frequencies in individuals; significance was set at P > 0.05.
There was an increase in both the MNC and MN frequency from habit controls to OPLs, indicating that the number of cells with chromosomal damage and extent of chromosomal damage in each cell was high in OPLs.
The MN count can be used as a noninvasive tool for early detection, educating patients, screening a large population, and to check the risk for malignancy, which in turn may help in treatment planning.
通过微核试验以及对这些癌前病变中生物标志物的定量和检测，研究烟草对口腔癌前病变（OPL）患者和有吸烟习惯但无口腔癌前病变的患者（习惯对照组）的颊黏膜脱落上皮细胞的遗传毒性作用，这将有助于找出那些发生恶性转化风险较高的患者。
从20名个体的颊黏膜上皮细胞的左右两侧采集40个样本，即10名习惯对照组患者和10名OPL患者。使用社会科学统计软件包21.0进行统计分析。采用非配对t检验确定个体中的微核细胞（MNC）和微核（MN）频率；显著性设定为P>0.05。
从习惯对照组到OPL患者，MNC和MN频率均增加，表明OPL中染色体受损细胞的数量以及每个细胞中染色体受损的程度都很高。
MN计数可用作早期检测、教育患者、筛查大量人群以及检查恶性风险的非侵入性工具，这反过来可能有助于治疗规划。
