Bansal Himanta, Sandhu V Simarpreet, Bhandari Rajat, Sharma Deepti
Department of Oral and Maxillofacial Pathology, Genesis Institute of Dental Sciences and Research, Ferozepur, Punjab, India.
Contemp Clin Dent. 2012 Apr;3(2):184-7. doi: 10.4103/0976-237X.96825.
The assessment of micronuclei in exfoliated cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis.
The present study aimed to detect micronuclei in exfoliated oral mucosal cells in individuals using various tobacco forms from the last 5 years.
A total of 75 healthy male subjects (25 smokeless tobacco users, 25 smokers, and 25 non-tobacco users) were selected for the study. Smears were obtained with moistened wooden spatula from buccal mucosa and fixed with 95% alcohol. All the cytologic smears were stained by Papanicolaou technique. From each slide, ~1000 cells were examined under the 400× magnification and where micronucleated (MN) cells were located, they were examined under the 1000× magnification.
MN cells were found to be significantly higher in smokeless tobacco users than in smokers and controls.
A positive correlation is found between increased micronucleus frequency and tobacco-using habits. So micronucleus assay can be used as a biomarker of genotoxicity.
评估脱落细胞中的微核是研究上皮致癌物的一种有前景的工具，可用于检测被认为与致癌作用相关的染色体断裂或有丝分裂干扰。
本研究旨在检测过去5年中使用各种烟草形式的个体口腔黏膜脱落细胞中的微核。
共选择75名健康男性受试者（25名无烟烟草使用者、25名吸烟者和25名非烟草使用者）进行研究。用湿润的木质压舌板从颊黏膜获取涂片，并用95%酒精固定。所有细胞学涂片采用巴氏染色技术染色。从每张载玻片上，在400倍放大倍数下检查约1000个细胞，对于发现有微核（MN）的细胞，在1000倍放大倍数下进行检查。
发现无烟烟草使用者中的MN细胞显著高于吸烟者和对照组。
微核频率增加与烟草使用习惯之间存在正相关。因此，微核试验可作为遗传毒性的生物标志物。
