Upadhyay Meenakshi, Verma Parul, Sabharwal Robin, Subudhi Santosh Kumar, Jatol-Tekade Suruchi, Naphade Vihang, Choudhury Basanta Kumar, Sahoo Pravudeva Devidutta
Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
Department of Conservative Dentistry and Endodontics, IDS, Sehora, Jammu, India.
Niger J Surg. 2019 Jan-Jun;25(1):52-59. doi: 10.4103/njs.NJS_10_18.
The aim of this study was to analyze the tobacco-related genotoxic effects in individual with habit of smoking and chewing tobacco.
The present study sample consisted of 120 individuals attending the outpatient department of D. J. College of Dental Sciences and Research, Modinagar, Uttar Pradesh (UP). The sample was divided into four groups as follows: Group I (individuals with habit of smoking tobacco), Group II (individuals with habit of chewing tobacco), Group III (individuals with habit of smoking and chewing tobacco), and Group IV control group (nontobacco-exposed individuals). Patients were asked to rinse their mouth gently with water. The exfoliated cells were obtained by scraping the buccal mucosa of individuals with a wooden spatula. The scraped cells were placed on the precleaned slides. The smears were then stained with RAPID-PAP™ and analyzed under the microscope. Data were analyzed using SPSS statistical software.
In the present study, an arbitrary unit was obtained using frequency/day multiplied by the duration of years (risk multiplication factor [RMF], a positive and significant correlation were observed between the RMF and the mean percentage of micronucleated cell count in smokers, chewers, and in individuals with both smoking and chewing habit, respectively. A weak positive and nonsignificant correlation were observed between age and mean percentage of micronucleated cells in smokers and smokers + chewers, respectively, while it was weak negative and nonsignificant in chewers. In control group, correlation between age and percentage of micronucleated cells was weak positive and nonsignificant at 5% level of significance.
The micronuclei in exfoliated mucosal cells from buccal mucosa can be used as a biomarker of genotoxicity in predicting the effects of carcinogens.
本研究旨在分析有吸烟和咀嚼烟草习惯的个体中与烟草相关的遗传毒性效应。
本研究样本包括120名就诊于印度北方邦莫迪纳加尔市DJ牙科学院及研究中心门诊部的个体。样本分为以下四组：第一组（有吸烟习惯的个体），第二组（有咀嚼烟草习惯的个体），第三组（有吸烟和咀嚼烟草习惯的个体），第四组为对照组（未接触烟草的个体）。要求患者用水轻轻漱口。通过用木制刮勺刮取个体的颊黏膜来获取脱落细胞。将刮取的细胞置于预先清洁过的载玻片上。然后用RAPID-PAP™进行染色，并在显微镜下分析。使用SPSS统计软件对数据进行分析。
在本研究中，通过频率/天乘以年数获得一个任意单位（风险倍增因子[RMF]），分别在吸烟者、咀嚼者以及有吸烟和咀嚼习惯的个体中观察到RMF与微核细胞计数的平均百分比之间存在正相关且具有统计学意义。在吸烟者和吸烟+咀嚼者中，年龄与微核细胞平均百分比之间分别观察到弱正相关且无统计学意义，而在咀嚼者中则为弱负相关且无统计学意义。在对照组中，年龄与微核细胞百分比之间的相关性在5%显著性水平下为弱正相关且无统计学意义。
颊黏膜脱落黏膜细胞中的微核可作为遗传毒性的生物标志物，用于预测致癌物的影响。
