Thomson G J, Vohra R, Walker M G
Department of Vascular Surgery, Manchester Royal Infirmary, United Kingdom.
Ann Vasc Surg. 1989 Apr;3(2):140-5. doi: 10.1016/S0890-5096(06)62007-4.
A series of experiments was performed to compare the ability of adult human endothelial and mesothelial cells to attach and spread upon ePTFE graft material. Both cell types were harvested enzymatically and grown in culture. Two types of adhesion assay were used to assess the ability of the cultured cells to attach to ePTFE either uncoated or precoated with a variety of substrate proteins. The results showed that fibronectin, laminin, type 4 collagen and preclotted blood all greatly improve the attachment rate of endothelium to ePTFE. The extracellular matrix proteins, however, give patchy cell attachment, whereas cells seeded onto preclotted ePTFE form a virtually confluent monolayer after one hour's incubation. The matrix proteins similarly improve mesothelial attachment, although in all cases attached cells remain largely rounded up, with relatively few spreading on the surface. Mesothelial attachment to preclotted ePTFE is no better than attachment to the untreated material. It would seem that a preclotted graft seeded with endothelial cells is the combination most likely to result in a confluent monolayer within one hour.
进行了一系列实验，以比较成人内皮细胞和间皮细胞在ePTFE移植材料上附着和铺展的能力。两种细胞类型均通过酶法收获并在培养物中生长。使用两种类型的粘附试验来评估培养细胞附着于未涂覆或预先涂覆有多种底物蛋白的ePTFE的能力。结果表明，纤连蛋白、层粘连蛋白、IV型胶原和预凝血液均能显著提高内皮细胞与ePTFE的附着率。然而，细胞外基质蛋白会使细胞呈斑块状附着，而接种到预凝ePTFE上的细胞在孵育一小时后会形成几乎汇合的单层。基质蛋白同样能改善间皮细胞的附着，尽管在所有情况下，附着的细胞大多仍呈圆形，在表面铺展的相对较少。间皮细胞与预凝ePTFE的附着并不比与未处理材料的附着更好。似乎接种了内皮细胞的预凝移植物最有可能在一小时内形成汇合的单层。
