Zhao Yan, Chen Jiao, Wang Xiu, Song Qi, Xu Hui-Hui, Zhang Yun-Hui
Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai, China.
Shanghai Municipal Center for Disease Control &Prevention, Shanghai, China.
Sci Rep. 2016 Sep 22;6:33449. doi: 10.1038/srep33449.
Strong evidence implicates maternal phthalate exposure during pregnancy in contributing to adverse birth outcomes. Recent research suggests these effects might be mediated through the improper regulation of DNA methylation in offspring tissue. In this study, we examined associations between prenatal phthalate exposure and DNA methylation in human placenta. We recruited 181 mother-newborn pairs (80 fetal growth restriction newborns, 101 normal newborns) in Wenzhou, China and measured third trimester urinary phthalate metabolite concentrations and placental DNA methylation levels of IGF2 and AHRR. We found urinary concentrations of mono (2-ethyl-5- hydroxyhexyl) phthalate (MEHHP), and mono (2-ethyl-5-oxohexyl) phthalate (MEOHP) were significantly inversely associated with placental IGF2 DNA methylation. The associations were much more evident in fetal growth restriction (FGR) newborns than those in normal newborns. These findings suggest that changes in placental DNA methylation might be part of the underlying biological pathway between prenatal phthalate exposure and adverse fetal growth.
有力证据表明，孕期母亲接触邻苯二甲酸盐会导致不良出生结局。近期研究表明，这些影响可能是通过子代组织中DNA甲基化的不当调控介导的。在本研究中，我们检测了产前邻苯二甲酸盐暴露与人类胎盘DNA甲基化之间的关联。我们在中国温州招募了181对母婴（80例胎儿生长受限新生儿，101例正常新生儿），并测量了孕晚期尿中邻苯二甲酸酯代谢物浓度以及胎盘组织中IGF2和AHRR的DNA甲基化水平。我们发现，尿中邻苯二甲酸单（2-乙基-5-羟基己基）酯（MEHHP）和邻苯二甲酸单（2-乙基-5-氧代己基）酯（MEOHP）的浓度与胎盘IGF2 DNA甲基化呈显著负相关。这种关联在胎儿生长受限（FGR）新生儿中比在正常新生儿中更为明显。这些发现表明，胎盘DNA甲基化的变化可能是产前邻苯二甲酸盐暴露与不良胎儿生长之间潜在生物学途径的一部分。
