Figueroa C D, MacIver A G, Bhoola K D
Department of Pharmacology Medical School, University of Bristol.
Br J Haematol. 1989 Jul;72(3):321-8. doi: 10.1111/j.1365-2141.1989.tb07711.x.
We have identified a tissue kallikrein in polymorphonuclear (PMN) leucocytes of normal human blood and bone marrow by immunocytochemistry, radioimmunoassay and enzymology. Immunoreactive tissue kallikrein was visualized in the mature neutrophil leucocytes and in immature forms such as metamyelocytes and myelocytes. No tissue kallikrein was detected in eosinophil leucocytes, lymphocytes, macrophages, megakaryocytes and platelets. So far, we have failed to observe immunoreactivity to tissue kallikrein in basophils. The presence of tissue kallikrein in extracts prepared from PMN leucocytes isolated from peripheral blood was demonstrated by immunodiffusion, dot-blotting and by radioimmunoassay. The kininogenase and amidase activity of the extracts resembled that of tissue kallikrein in being resistant to soya bean trypsin inhibitor and sensitive to trasylol. The amidase activity attributable to tissue kallikrein was completely inhibited by specific antisera.
我们通过免疫细胞化学、放射免疫测定和酶学方法，在正常人血液和骨髓的多形核（PMN）白细胞中鉴定出一种组织激肽释放酶。在成熟的中性粒细胞以及未成熟形式如晚幼粒细胞和中幼粒细胞中可观察到免疫反应性组织激肽释放酶。在嗜酸性粒细胞、淋巴细胞、巨噬细胞、巨核细胞和血小板中未检测到组织激肽释放酶。到目前为止，我们未能在嗜碱性粒细胞中观察到对组织激肽释放酶的免疫反应性。通过免疫扩散、斑点印迹和放射免疫测定证实，从外周血分离的PMN白细胞制备的提取物中存在组织激肽释放酶。提取物的激肽原酶和酰胺酶活性与组织激肽释放酶相似，对大豆胰蛋白酶抑制剂有抗性，对抑肽酶敏感。归因于组织激肽释放酶的酰胺酶活性可被特异性抗血清完全抑制。
