Sun Junqing, Xie Wenjun, Shi Liang, Yu Liyin, Zhang Jianbao
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
Institute of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
Biomech Model Mechanobiol. 2017 Apr;16(2):509-520. doi: 10.1007/s10237-016-0833-y. Epub 2016 Sep 21.
Intracellular [Formula: see text] transient induced by fluid shear stress (FSS) plays an important role in mechanical regulation of osteoblasts, but the cellular mechanism remains incompletely understood. Here, we constructed a mathematical model combined with experiments to elucidate it. Our simulated and experimental results showed that it was the delay of membrane potential repolarization to produce the refractory period of FSS-induced intracellular calcium transients in osteoblasts. Moreover, the results also demonstrated that the amplitude of FSS-induced intracellular calcium transient is crucial to the proliferation, while its duration is critical to the differentiation, of osteoblasts. Overall, the present study provides a way to understand the cellular mechanism of intracellular calcium transients in osteoblast induced by FSS and explains some of related physiological events.
流体剪切应力（FSS）诱导的细胞内[公式：见正文]瞬变在成骨细胞的机械调节中起重要作用，但其细胞机制仍未完全阐明。在此，我们构建了一个结合实验的数学模型来阐明这一机制。我们的模拟和实验结果表明，是膜电位复极化的延迟产生了FSS诱导的成骨细胞内钙瞬变的不应期。此外，结果还表明，FSS诱导的细胞内钙瞬变的幅度对成骨细胞的增殖至关重要，而其持续时间对成骨细胞的分化至关重要。总体而言，本研究提供了一种理解FSS诱导的成骨细胞内钙瞬变细胞机制的方法，并解释了一些相关的生理事件。
