Meng Guixian, Li Cunbo, Sun Haiying, Lee Imshik
The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Institute of Applied Physics and School of Physics, Nankai University, Tianjin, China.
Academy of Laboratory, Jilin Medical University, Jilin, China.
J Orthop Res. 2018 Jul;36(7):2039-2051. doi: 10.1002/jor.23843. Epub 2018 Jan 10.
The intracellular calcium ([Ca ] ) response induced by external forces can be diverse and complex. Using primary osteoblasts from Wistar rats, we found multiple patterns of [Ca ] responses induced by fluidic shear stress (Fss), including homogeneous non-oscillation and heterogeneous oscillations. These multiple-patterned [Ca ] responses could be influenced by Fss intensity, cell density, and cell differentiation. Our real-time measurements with free calcium, ATP, ATP without calcium, suramin, apyrase, and thapsigargin confirmed homogeneous [Ca ] patterns and/or heterogeneous [Ca ] oscillations with respect to the combined degree of external Ca influx, and intracellular Ca release. Our theoretical model supported diverse Fss-induced calcium activities as well. We concluded that a singular factor of Ca influx or release dominated to produce smooth homogeneous patterns, but combined factors produced oscillatory heterogeneous patterns. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2039-2051, 2018.
外力诱导的细胞内钙（[Ca]）反应可能多种多样且复杂。利用来自Wistar大鼠的原代成骨细胞，我们发现了流体剪切力（Fss）诱导的多种[Ca]反应模式，包括均匀非振荡和异质振荡。这些多模式的[Ca]反应可能受Fss强度、细胞密度和细胞分化的影响。我们使用游离钙、ATP、无钙ATP、苏拉明、腺苷三磷酸双磷酸酶和毒胡萝卜素进行的实时测量，证实了关于外部钙内流和细胞内钙释放的综合程度的均匀[Ca]模式和/或异质[Ca]振荡。我们的理论模型也支持多种Fss诱导的钙活性。我们得出结论，单一的钙内流或释放因素主导产生平滑的均匀模式，但多种因素共同作用产生振荡的异质模式。©2017骨科学研究协会。由Wiley Periodicals, Inc.出版。《矫形外科学研究》36:2039 - 2051, 2018。
