Tong Jie, Sun Lijun, Zhu Bin, Fan Yun, Ma Xingfeng, Yu Liyin, Zhang Jianbao
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.
Xi Jing University, Xi'an, China.
Bioelectromagnetics. 2017 Oct;38(7):541-549. doi: 10.1002/bem.22076. Epub 2017 Aug 18.
Pulsed electromagnetic fields (PEMF) can be used to treat bone-related diseases, but the underlying mechanism remains unclear, especially the process by which PEMFs initiate biological effects. In this study, we demonstrated the effects of PEMF on proliferation and differentiation of osteoblasts using the model of calcium transients induced by high extracellular calcium. Our results showed that PEMF can increase both the percentage of responding cells and amplitude of intracellular calcium transients induced by high extracellular calcium stimulation. Compared with corresponding extracellular calcium levels, PEMF stimulation increased proliferation and differentiation of osteoblasts and related gene expressions, such as insulin-like growth factor 1 (IGF-1), alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteocalcin (OCN), which can be completely abolished by BAPTA-AM. Moreover, PEMF did not affect proliferation and differentiation of osteoblasts if no intracellular calcium transient was present in osteoblasts during PEMF exposure. Our results revealed that PEMF affects osteoblast proliferation and differentiation through enhanced intracellular calcium transients, which provided a cue to treat bone-related diseases with PEMF. Bioelectromagnetics. 38:541-549, 2017. © 2017 Wiley Periodicals, Inc.
脉冲电磁场（PEMF）可用于治疗与骨骼相关的疾病，但其潜在机制仍不清楚，尤其是PEMF引发生物学效应的过程。在本研究中，我们使用高细胞外钙诱导的钙瞬变模型，证明了PEMF对成骨细胞增殖和分化的影响。我们的结果表明，PEMF可增加高细胞外钙刺激诱导的反应细胞百分比和细胞内钙瞬变幅度。与相应的细胞外钙水平相比，PEMF刺激增加了成骨细胞的增殖和分化以及相关基因表达，如胰岛素样生长因子1（IGF-1）、碱性磷酸酶（ALP）、 runt相关转录因子2（Runx2）和骨钙素（OCN），而BAPTA-AM可完全消除这些作用。此外，如果在PEMF暴露期间成骨细胞中不存在细胞内钙瞬变，PEMF不会影响成骨细胞的增殖和分化。我们的结果表明，PEMF通过增强细胞内钙瞬变来影响成骨细胞的增殖和分化，这为用PEMF治疗与骨骼相关的疾病提供了线索。生物电磁学。38:541 - 549，2017年。©2017威利期刊公司。
