Balmukhanov B S, Basenova A T, Bulegenov K E
Biofizika. 1989 May-Jun;34(3):468-72.
The sedimentation rates (SR) of human red blood cells (RBC) were measured in diluted suspensions using the thin plate chamber. If the suspension medium was phosphate buffer saline or 0,18 M NaCl the SR-dependence on the distance to the chambers wall corresponded to SR distribution of small particles without interaction. The more NaCl content was decreased down to 0.145 M, the more temperature-dependent variations of SR were noted, while SR distribution became distinct with the predicted one for the non-interacting particles. The use of SR distribution is discussed for testing the RBC interaction in diluted suspensions caused by sedimentation. The electrophoretic measurements carried out under vertical oriented electrical field showed the rate of RBC movement to be the linear function of the field gradient and to be not influenced under the SR modifying conditions.
使用薄板腔室在稀释悬浮液中测量人红细胞(RBC)的沉降速率(SR)。如果悬浮介质是磷酸盐缓冲盐水或0.18M NaCl,则SR对腔室壁距离的依赖性对应于无相互作用的小颗粒的SR分布。NaCl含量越低至0.145M,SR的温度依赖性变化就越明显,而SR分布与非相互作用颗粒的预测分布变得不同。讨论了使用SR分布来测试由沉降引起的稀释悬浮液中RBC的相互作用。在垂直取向电场下进行的电泳测量表明,RBC的移动速率是场梯度的线性函数,并且在SR改变条件下不受影响。