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[氯扩散电位对稀释悬浮液中电泳迁移率和红细胞沉降的影响]

[The effect of chlorine diffusion potential on electrophoretic mobility and erythrocyte sedimentation in diluted suspensions].

作者信息

Balmukhanov B, Basenova A

出版信息

Biofizika. 1994 Jul-Aug;39(4):686-90.

PMID:7981276
Abstract

Electrophoretic activity of human erythrocytes in isotonic medium with reduced NaCl content does not depend on the outer electric field strength when pH decreases from 7.3 to 5.5. At pH approximately equal to 5.3 the measured values of electrophoretic mobility drop with decrease of the outer electric field strength starting at 600-700 V/m. Simultaneously signs of the solitary settlement of erythrocytes diluted suspensions are disturbed. Estimated values of the chlorine diffusion potential in these conditions exceed the value 70-80 mV, at which the erythrocyte membrane permeability changes. It is suggested that the observed dependency of electrophoretic mobility on the field strength is caused by ion flows through the erythrocyte membrane leading to diffusion non-equilibrium in the electrolyte layer close to the membrane.

摘要

当pH值从7.3降至5.5时,在NaCl含量降低的等渗介质中,人红细胞的电泳活性不依赖于外部电场强度。在pH值约等于5.3时,从600 - 700 V/m开始,随着外部电场强度的降低,测得的电泳迁移率值下降。同时,红细胞稀释悬浮液的单独沉降迹象受到干扰。在这些条件下,氯扩散电位的估计值超过70 - 80 mV,此时红细胞膜通透性发生变化。有人认为,观察到的电泳迁移率对场强的依赖性是由离子流经红细胞膜导致膜附近电解质层扩散不平衡引起的。

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