金黄色葡萄球菌和表皮葡萄球菌在骨科植入材料上的体外和体内比较定植情况

In vitro and in vivo comparative colonization of Staphylococcus aureus and Staphylococcus epidermidis on orthopaedic implant materials.

作者信息

Barth E, Myrvik Q M, Wagner W, Gristina A G

机构信息

Section of Orthopedic Surgery, Bowman Gray School of Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27103.

出版信息

Biomaterials. 1989 Jul;10(5):325-8. doi: 10.1016/0142-9612(89)90073-2.

DOI:10.1016/0142-9612(89)90073-2

PMID:2765629

Abstract

Clinically, Staphylococcus aureus appears to be the dominant organism associated with infected metal implants, whereas coagulase-negative staphylococcal strains are more frequently isolated from infected polymer implants. We reproduced this trend experimentally in vitro and in vivo. Discs of a titanium alloy, poly(methyl methacrylate) and ultra-high molecular weight polyethylene were exposed to a clinical isolate of Staphylococcus aureus or either of two strains of Staphylococcus epidermidis. Within 1 h Staphylococcus aureus was always the most rapid colonizer regardless of biomaterial. However, after 8 to 24 h, Staphylococcus aureus was present in higher numbers on metal and Staphylococcus epidermidis on polymers. Moreover, the exopolysaccharide produced by Staphylococcus epidermidis appeared to offer an effective protection against host defences in vivo.

摘要

临床上，金黄色葡萄球菌似乎是与感染的金属植入物相关的主要病原体，而凝固酶阴性葡萄球菌菌株则更频繁地从感染的聚合物植入物中分离出来。我们在体外和体内实验中重现了这一趋势。将钛合金、聚甲基丙烯酸甲酯和超高分子量聚乙烯圆盘暴露于金黄色葡萄球菌的临床分离株或两种表皮葡萄球菌菌株之一。在1小时内，无论生物材料如何，金黄色葡萄球菌始终是最快速的定植菌。然而，在8至24小时后，金属上的金黄色葡萄球菌数量更多，聚合物上的表皮葡萄球菌数量更多。此外，表皮葡萄球菌产生的胞外多糖似乎在体内对宿主防御提供了有效的保护。