Bihn Soo Kyum, Son Keunbada, Son Young-Tak, Dahal Ram Hari, Kim Shukho, Kim Jungmin, Hwang Jun Ho, Kwon Sung-Min, Lee Jong Hoon, Kim Hyun Deok, Lee Jae-Mok, Jin Myoung-Uk, Lee Kyu-Bok
Department of Prosthodontics, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea.
Advanced Dental Device Development Institute (A3DI), Kyungpook National University, Daegu 41940, Republic of Korea.
J Funct Biomater. 2023 Sep 22;14(10):486. doi: 10.3390/jfb14100486.
(1) Background: The purpose of this study was to evaluate how a zirconia implant surface treated with laser technology affects the degree of biofilm formation. (2) Methods: Experimental titanium (Ti) disks were produced that were sandblasted with large grit and acid-etched (T), and they were compared with zirconia (ZrO) discs with a machined (M) surface topography; a hydrophilic surface topography with a femtosecond laser (HF); and a hydrophobic surface topography with a nanosecond laser (HN) (N = 12 per surface group). An in vitro three-species biofilm sample (Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi)) was applied to each disc type, and bacterial adhesion was assessed after 48 and 72 h of incubation using an anaerobic flow chamber model. Statistical significance was determined using the Kruskal-Wallis H test, with Bonferroni correction used for the post-hoc test (α = 0.05). (3) Results: Compared to the T group, the M group exhibited more than twice as many viable bacterial counts in the three-species biofilm samples ( < 0.05). In comparison to the T group, the HF group had significantly higher viable bacterial counts in certain biofilm samples at 48 h (Aa and Pi) and 72 h (Pi) ( < 0.05). The HN group had higher viable bacterial counts in Pi at 48 h (5400 CFU/mL, < 0.05) than the T group (4500 CFU/mL), while showing significantly lower viable bacterial counts in Pg at both 48 (3010 CFU/mL) and 72 h (3190 CFU/mL) ( < 0.05). (4) Conclusions: The surface treatment method for zirconia discs greatly influences biofilm formation. Notably, hydrophobic surface treatment using a nanosecond laser was particularly effective at inhibiting Pg growth.
(1) 背景：本研究的目的是评估经激光技术处理的氧化锆种植体表面如何影响生物膜形成程度。(2) 方法：制作了经大颗粒喷砂和酸蚀处理的实验性钛（Ti）盘（T），并将其与具有机械加工（M）表面形貌的氧化锆（ZrO）盘、具有飞秒激光处理的亲水性表面形貌（HF）的氧化锆盘以及具有纳秒激光处理的疏水性表面形貌（HN）的氧化锆盘进行比较（每个表面组N = 12）。将体外三种细菌生物膜样本（伴放线聚集杆菌（Aa）、牙龈卟啉单胞菌（Pg）、中间普氏菌（Pi））应用于每种盘类型，并使用厌氧流动腔模型在孵育48小时和72小时后评估细菌粘附情况。使用Kruskal-Wallis H检验确定统计学显著性，并使用Bonferroni校正进行事后检验（α = 0.05）。(3) 结果：与T组相比，M组在三种细菌生物膜样本中的活菌计数多出两倍以上（<0.05）。与T组相比，HF组在48小时（Aa和Pi）和72小时（Pi）的某些生物膜样本中活菌计数显著更高（<0.05）。HN组在48小时时Pi的活菌计数（5400 CFU/mL，<0.05）高于T组（4500 CFU/mL），而在48小时（3010 CFU/mL）和72小时（3190 CFU/mL）时Pg的活菌计数均显著低于T组（<0.05）。(4) 结论：氧化锆盘的表面处理方法对生物膜形成有很大影响。值得注意的是，使用纳秒激光进行疏水性表面处理在抑制Pg生长方面特别有效。
