Pajares-Chamorro Natalia, Wagley Yadav, Maduka Chima V, Youngstrom Daniel W, Yeger Alyssa, Badylak Stephen F, Hammer Neal D, Hankenson Kurt, Chatzistavrou Xanthippi
Department of Chemical Engineering and Materials Science, College of Engineering, Michigan State University, East Lansing, MI 48824, USA.
Department of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, MI 48103, USA.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111693. doi: 10.1016/j.msec.2020.111693. Epub 2020 Nov 5.
Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.
感染是影响骨骼及其他组织愈合失败的一个重要风险因素。我们研发了一种通过溶胶-凝胶（溶液-凝胶化）法制备的、掺杂银离子的生物活性玻璃（Ag-BG），旨在提供无细胞毒性的抗菌活性，同时在体外显著促进成骨细胞系细胞生长，并在体内促进骨再生。我们的目标是设计一种生物材料，既能对抗细菌感染，又能保持促进骨生长的能力。我们观察到，Ag-BG可抑制细菌生长，并增强传统抗生素治疗的效果。Ag-BG微粒在体外可增强人骨髓基质细胞（hBMSC）的细胞增殖和成骨分化。此外，利用小鼠颅骨缺损模型进行的体内试验表明，Ag-BG微粒可诱导骨再生。这种具有双重生物学特性和先进抗菌性能的新型系统，是一种对抗耐药菌并引发新骨形成的很有前景的治疗方法。
