Yang Xuesong, Fu Timothy, Kota Pavan Kumar, Tjia Maggie, Nguyen Cuong Manh, Chiao Jung-Chih
Electrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USA.
Texas A&M Health Science Center, Bryan, TX 77807, USA.
Biosensors (Basel). 2016 Sep 21;6(3):48. doi: 10.3390/bios6030048.
Lactate detection by an in situ sensor is of great need in clinical medicine, food processing, and athletic performance monitoring. In this paper, a flexible, easy to fabricate, and low-cost biosensor base on lactate oxidase is presented. The fabrication processes, including metal deposition, sol-gel IrO deposition, and drop-dry enzyme loading method, are described in detail. The loaded enzyme was examined by scanning electron microscopy. Cyclic voltammetry was used to characterize the sensors. Durability, sensibility, and selectivity of the biosensors were examined. The comparison for different electrode sizes and different sensing film materials was conducted. The sensor could last for four weeks with an average surface area normalized sensitivity of 950 nA/(cm² mM) and 9250 nA/(cm² mM) for Au-based electrodes, and IrO-modified electrodes respectively, both with an electrode size of 100 × 50 μm. The self-referencing method to record noises simultaneously with the working electrode greatly improved sensor sensitivity and selectivity. The sensor showed little response to interference chemicals, such as glutamate and dopamine.
通过原位传感器检测乳酸在临床医学、食品加工和运动表现监测中具有迫切需求。本文介绍了一种基于乳酸氧化酶的柔性、易于制造且低成本的生物传感器。详细描述了包括金属沉积、溶胶 - 凝胶法沉积氧化铱以及滴干法负载酶在内的制造工艺。通过扫描电子显微镜对负载的酶进行了检测。使用循环伏安法对传感器进行表征。检测了生物传感器的耐久性、灵敏度和选择性。对不同电极尺寸和不同传感膜材料进行了比较。对于尺寸为100×50μm的金基电极和氧化铱修饰电极，该传感器分别可维持四周，平均表面积归一化灵敏度分别为950 nA/(cm² mM)和9250 nA/(cm² mM)。与工作电极同时记录噪声的自参考方法极大地提高了传感器的灵敏度和选择性。该传感器对干扰化学物质（如谷氨酸和多巴胺）几乎没有响应。
