Pan Xueying, Lu Liang, Zeng Xiancheng, Chang Yan, Hua Xiuguo
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China; National Shanghai Center for New Drug Safety Evaluation and Research (NCDSER), Shanghai, China.
National Shanghai Center for New Drug Safety Evaluation and Research (NCDSER), Shanghai, China.
J Am Assoc Lab Anim Sci. 2016;55(5):534-40.
To evaluate the effects of transportation on oxidative stress in cynomolgus monkeys, we measured serum levels of reduced glutathione (GSH), malondialdehyde, and protein carbonyl (PC) and the activities of total antioxidant capacity (TAOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase in cynomolgus macaques before transportation (day 0), on the day of arrival (day 1), and on days 7, 14, and 21 after transportation. Compared with that on day 0, TAOC and catalase activities on days 1, 7, and 14 after transportation were significantly decreased, reached their nadirs on day 7, and increased thereafter to reach their pretransportation levels by day 21 after transportation. Compared with day 0 levels, mean SOD activity and GSH concentration were decreased significantly on day 1; they thereafter increased to reach their pretransportation measures by day 7 after transportation. In contrast, PC and malondialdehyde concentrations in serum and the activity of GSH-Px were increased on day 1 compared with day 0 and thereafter decreased to reach their pretransportation levels by day 14 after transportation. In summary, GSH, TAOC, catalase, and SOD levels decreased and malondialdehyde, PC, and GSH-Px concentrations increased in cynomolgus macaques after transportation. These results suggest that transportation might imbalance oxidant and antioxidant levels to create excess oxidative stress in cynomolgus macaques. Therefore, cynomolgus macaques should have at least 21 d to recover after transportation and regain their healthy status.
为了评估运输对食蟹猴氧化应激的影响，我们测量了食蟹猴在运输前（第0天）、到达当天（第1天）以及运输后第7天、第14天和第21天血清中还原型谷胱甘肽（GSH）、丙二醛和蛋白质羰基（PC）的水平，以及总抗氧化能力（TAOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶的活性。与第0天相比，运输后第1天、第7天和第14天的TAOC和过氧化氢酶活性显著降低，在第7天达到最低点，此后升高，到运输后第21天恢复到运输前水平。与第0天的水平相比，第1天的平均SOD活性和GSH浓度显著降低；此后升高，到运输后第7天恢复到运输前的水平。相比之下，与第0天相比，第1天血清中的PC和丙二醛浓度以及GSH-Px的活性升高，此后降低，到运输后第14天恢复到运输前水平。总之，运输后食蟹猴的GSH、TAOC、过氧化氢酶和SOD水平降低，丙二醛(1,3),PC和GSH-Px浓度升高。这些结果表明，运输可能会使氧化剂和抗氧化剂水平失衡，从而在食蟹猴中产生过量的氧化应激。因此，食蟹猴在运输后至少应有21天的恢复时间，以恢复其健康状态。
