College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Zhejiang University, 310058, Hangzhou, Zhejiang, China; Department of Food Science, Cornell University, 14853-7201, Ithaca, NY, USA.
Department of Food Science, Cornell University, 14853-7201, Ithaca, NY, USA.
Fish Shellfish Immunol. 2018 Jan;72:564-571. doi: 10.1016/j.fsi.2017.11.016. Epub 2017 Nov 11.
Low temperature and air exposure were the key attributes for waterless transportation of fish and shrimp. In order to investigate the oxidative stress and antioxidant responses of the live shrimp Litopenaeus vannamei in the mimic waterless transportation, live shrimp were cooled at 13 °C for 3 min, stored in oxygen at 15 °C for 12 h, and then revived in water at 25 °C. The survival rate of shrimp under this waterless transportation system was over 86.67%. The ultrastructure of hepatopancreas cells were observed while activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione peroxidase (GSH-Px), antisuperoxide anion free radicals (ASAFR), total antioxidant capacity (TAOC), reactive oxygen species (ROS) production, content of malondialdehyde (MDA) and relative mRNA expressions of CAT and GSH-Px in the hemolymph and hepatopancreas were determined. Slight distortions of some organelles in hepatopancreas cells was reversible upon the shrimp revived from the cold shock. The activities of SOD, POD, CAT, GSH-Px, TAOC, ROS production and relative mRNA expressions of CAT and GSH-Px increased following the cold shock and reached peak levels after 3 or 6 h of storage, and then decreased gradually. There was no significant difference between the fresh and the revived shrimp in SOD, POD, GSH-Px, TAOC, ROS, MDA and relative mRNA expressions of CAT and GSH-Px. The oxidative stress and antioxidant responses were tissue-specific because hepatopancreas seemed to have a greater ability to defend against organelle damage and was more sensitive to stress than hemolymph based on the results of SOD activity, MDA content and GSH-Px mRNA expression. These results revealed that low temperature and air exposure caused significant oxidative and antioxidant responses, but did not lead to irreversible damages in this waterless system.
低温和空气暴露是无水运输鱼类和虾类的关键属性。为了研究模拟无水运输过程中活对虾凡纳滨对虾的氧化应激和抗氧化反应，将活虾在 13°C 下冷却 3 分钟，在 15°C 的氧气中储存 12 小时，然后在 25°C 的水中复苏。在这种无水运输系统下，虾的存活率超过 86.67%。观察了肝胰腺细胞的超微结构，同时测定了超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、抗超氧阴离子自由基（ASAFR）、总抗氧化能力（TAOC）、活性氧（ROS）产生、丙二醛（MDA）含量以及血淋巴和肝胰腺中 CAT 和 GSH-Px 的相对 mRNA 表达。虾从冷休克中复苏后，肝胰腺细胞中一些细胞器的轻微扭曲是可逆的。冷休克后 SOD、POD、CAT、GSH-Px、TAOC、ROS 产生和 CAT 和 GSH-Px 的相对 mRNA 表达增加，在储存 3 或 6 小时后达到峰值，然后逐渐下降。新鲜虾和复苏虾在 SOD、POD、GSH-Px、TAOC、ROS、MDA 和 CAT 和 GSH-Px 的相对 mRNA 表达方面没有显著差异。氧化应激和抗氧化反应是组织特异性的，因为根据 SOD 活性、MDA 含量和 GSH-Px mRNA 表达的结果，肝胰腺似乎具有更大的抵御细胞器损伤的能力，并且比血淋巴对压力更敏感。这些结果表明，在这种无水系统中，低温和空气暴露会导致显著的氧化和抗氧化反应，但不会导致不可逆转的损伤。
