Ookuma K, Yoshimatsu H, Sakata T, Fujimoto K, Fukagawa F
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Brain Res. 1989 Jun 26;490(2):268-75. doi: 10.1016/0006-8993(89)90244-8.
To identify sites of histaminergic modulation of food intake, histamine H1-receptor antagonist was microinfused into the rat hypothalamus, the ventromedial hypothalamus (VMH), the lateral hypothalamus (LHA), the paraventricular nucleus (PVN), the dorsomedial hypothalamus (DMH), or the preoptic anterior hypothalamus (POAH), during the early light period. Feeding, but not drinking, was elicited in 100% of the rats (P less than 0.01) that were bilaterally microinfused with 26 nmol chlorpheniramine into the VMH. Unilateral infusion into the VMH did not affect food intake at doses of 26 or 52 nmol. Feeding was also induced by bilateral microinfusion into the PVN, but only the 52 nmol dose was effective. Bilateral infusions into the LHA, the DMH or the POAH did not affect ingestive behavior. Feeding induced by an H1-antagonist was completely abolished in all 7 rats tested when endogenous neuronal histamine was decreased by pretreatment with alpha-fluoromethylhistidine (100 mg/kg). The findings suggest that H1-receptors in the VMH and the PVN, but not in the LHA, the DMH or the POAH, may be involved in histaminergic suppression of food intake.
为了确定组胺能对食物摄入进行调节的部位，在光照早期，将组胺H1受体拮抗剂微量注入大鼠下丘脑、腹内侧下丘脑（VMH）、外侧下丘脑（LHA）、室旁核（PVN）、背内侧下丘脑（DMH）或视前区下丘脑前部（POAH）。向VMH双侧微量注入26 nmol氯苯那敏的大鼠中，100%出现进食行为，但饮水行为未出现（P<0.01）。向VMH单侧注入26或52 nmol剂量时，不影响食物摄入。向PVN双侧微量注入也可诱导进食，但仅52 nmol剂量有效。向LHA、DMH或POAH双侧注入不影响摄食行为。在用α-氟甲基组胺（100 mg/kg）预处理使内源性神经元组胺减少后，所有7只受试大鼠中，由H1拮抗剂诱导的进食行为均完全消失。这些发现表明，VMH和PVN中的H1受体，而非LHA、DMH或POAH中的H1受体，可能参与了组胺能对食物摄入的抑制作用。
