Sawada M, Kondo N, Suzumura A, Marunouchi T
Division of Cell Biology, Fujita-Gakuen Health University, Aichi, Japan.
Brain Res. 1989 Jul 10;491(2):394-7. doi: 10.1016/0006-8993(89)90078-4.
Stimulation of mouse astrocytes and microglia in enriched culture by lipopolysaccharide (LPS) results in the production of a cytotoxic factor that is functionally similar to macrophage-derived tumor necrosis factor-alpha (TNF). This cytotoxic factor kills murine L929 cells, and is neutralized by a monoclonal anti-mouse TNF. The production of this factor is inhibited by pretreatment of cells by actinomycin D. Microglia are more sensitive to LPS and produce much higher activity of cytotoxicity in lower concentration of LPS than astrocytes. These results suggest that microglia are the major producers of TNF in the brain and may play a role in certain pathological conditions in the brain.
在富集培养中，脂多糖（LPS）刺激小鼠星形胶质细胞和小胶质细胞会导致产生一种细胞毒性因子，其功能类似于巨噬细胞衍生的肿瘤坏死因子-α（TNF）。这种细胞毒性因子可杀死鼠L929细胞，并被单克隆抗小鼠TNF中和。放线菌素D预处理细胞可抑制该因子的产生。小胶质细胞对LPS更敏感，在较低浓度的LPS下比星形胶质细胞产生更高的细胞毒性活性。这些结果表明，小胶质细胞是脑中TNF的主要产生者，可能在脑的某些病理状况中起作用。
