Chiang C S, McBride W H
Department of Radiation Oncology, UCLA Medical Center 90024-1714.
Brain Res. 1991 Dec 6;566(1-2):265-9. doi: 10.1016/0006-8993(91)91707-8.
Astrocytes and microglial cells cultured from murine brain were stimulated to produce tumor necrosis factor alpha (TNF) by exposure to lipopolysaccharide (LPS). TNF alpha production began within 2 h with maximum production between 4 and 8 h after stimulation. Clinically relevant low (2 Gy), but not high (8 Gy), doses of radiation significantly increased TNF production by astrocytes and microglial cells in response to LPS. The radiation effect was even more marked with multiple 2 Gy doses. TNF is cytotoxic for oligodendrocytes and for certain tumor cells. It increases vascular permeability and enhances immune responses as well as having other biological effects. It is conceivable that production of TNF by astrocytes and microglial cells during clinical radiation therapy might influence the responses of tumor and/or normal CNS tissues.
从小鼠大脑培养的星形胶质细胞和小胶质细胞通过暴露于脂多糖（LPS）被刺激产生肿瘤坏死因子α（TNF）。TNFα的产生在2小时内开始，刺激后4至8小时达到最大产量。临床相关的低剂量（2 Gy）而非高剂量（8 Gy）辐射显著增加了星形胶质细胞和小胶质细胞对LPS反应时的TNF产生。多次2 Gy剂量的辐射效应更为明显。TNF对少突胶质细胞和某些肿瘤细胞具有细胞毒性。它增加血管通透性，增强免疫反应以及具有其他生物学效应。可以想象，临床放射治疗期间星形胶质细胞和小胶质细胞产生的TNF可能会影响肿瘤和/或正常中枢神经系统组织的反应。
