Qian Tian, Chen Fangru, Shi Xiaowei, Li Jian, Li Min, Chen Yan, Hao Fei, Zhang Dongmei
a Department of Dermatology , Southwest Hospital, Third Military Medical University , Chongqing , P.R. China.
b Department of Dermatology , Affiliated Hospital of Guilin Medical University , Guilin , P.R. China.
Mod Rheumatol. 2017 Jul;27(4):657-663. doi: 10.1080/14397595.2016.1232331. Epub 2016 Sep 23.
We aimed to examine CCAAT/enhancer-binding protein β (C/EBP β), TNF-alpha-induced protein 3 (TNFAIP3), and TNFAIP3-interacting protein 1 (TNIP1) expression in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients to assess their relationship in SLE pathogenesis.
C/EBP β, TNIP1, and TNFAIP3 expression was assessed in PBMCs from 20 SLE patients and 20 controls by western blotting. The correlation between C/EBP β/TNFAIP3/TNIP1 expression and SLE disease activity was determined by Spearman's rank. C/EBP β, TNIP1, and TNFAIP3 levels in THP-1 cells, THP-1 cells transfected with plasmids encoding TNFAIP3 shRNA, and THP-1 cells infected with lentiviral vectors encoding TNIP1 shRNA were assessed by western blotting.
C/EBP β LAP isoform expression was increased and LIP/TNFAIP3/TNIP1 expression was decreased in SLE patients. LAP expression was positively correlated with SLE disease activity; TNFAIP3 and TNIP1 expression was negatively correlated with SLE disease activity. LAP expression was increased in SLE patients with proteinuria and elevated anti-dsDNA antibody, as well as in THP-1 cells transfected with plasmids encoding TNFAIP3 shRNA and THP-1 cells infected with lentiviral vectors encoding TNIP1 shRNA.
C/EBP β/TNFAIP3/TNIP1 is associated with SLE activity. The upregulated expression of C/EBP β LAP could be caused by reduced TNFAIP3/TNIP1 expression.
我们旨在检测系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中CCAAT/增强子结合蛋白β（C/EBPβ）、肿瘤坏死因子α诱导蛋白3（TNFAIP3）和TNFAIP3相互作用蛋白1（TNIP1）的表达，以评估它们在SLE发病机制中的关系。
通过蛋白质印迹法检测20例SLE患者和20例对照者PBMC中C/EBPβ、TNIP1和TNFAIP3的表达。采用Spearman秩相关分析确定C/EBPβ/TNFAIP3/TNIP1表达与SLE疾病活动度之间的相关性。通过蛋白质印迹法评估THP-1细胞、转染编码TNFAIP3短发夹RNA（shRNA）质粒的THP-1细胞以及感染编码TNIP1 shRNA慢病毒载体的THP-1细胞中C/EBPβ、TNIP1和TNFAIP3的水平。
SLE患者中C/EBPβ的LAP异构体表达增加，而LIP/TNFAIP3/TNIP1表达降低。LAP表达与SLE疾病活动度呈正相关；TNFAIP3和TNIP1表达与SLE疾病活动度呈负相关。蛋白尿和抗双链DNA抗体升高的SLE患者以及转染编码TNFAIP3 shRNA质粒的THP-1细胞和感染编码TNIP1 shRNA慢病毒载体的THP-1细胞中LAP表达增加。
C/EBPβ/TNFAIP3/TNIP1与SLE活动度相关。C/EBPβ LAP表达上调可能是由于TNFAIP3/TNIP1表达降低所致。
