Qian Tian, Chen Yan, Shi Xiaowei, Li Jian, Hao Fei, Zhang Dongmei
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
Exp Ther Med. 2016 Oct;12(4):2348-2354. doi: 10.3892/etm.2016.3612. Epub 2016 Aug 23.
CCAAT/enhancer-binding protein β (C/EBP β) has important roles in numerous signaling pathways. The expression of the majority of regulators and target gene products of C/EBP β, including tumor necrosis factor α-induced protein 3 () and TNFAIP3-interacting protein 1 (), are upregulated in patients with systemic lupus erythematosus (SLE). The aim of the present study was to investigate whether / β expression is associated with SLE pathogenesis and correlates with and expression. Quantitative reverse transcription-polymerase chain reaction analysis was used to assess the expression of / β, , and mRNA in peripheral blood mononuclear cells (PBMC) from 20 patients with SLE and 20 healthy controls. Spearman's rank test was used to determine the correlation between / β expression and SLE disease activity, and that between / β expression and / expression in PBMCs from patients with SLE. / β mRNA expression was markedly increased in patients with SLE compared with healthy controls. The expression of / β was positively correlated with the SLE disease activity index and negatively correlated with the serum level of complement components C3 and C4. In addition, β mRNA expression was increased in PBMCs from SLE patients that were positive for antinuclear, anti-Smith and anti-nRNP antibodies, compared with the antibody negative SLE patients. Furthermore, the mRNA expression levels of / β in patients with SLE was positively correlated with and expression. The results of the current study suggest that the increased expression of / β in PBMCs and the interaction between / β and / may be involved in the pathogenesis of SLE.
CCAAT/增强子结合蛋白β（C/EBPβ）在众多信号通路中发挥重要作用。C/EBPβ的大多数调节因子和靶基因产物的表达，包括肿瘤坏死因子α诱导蛋白3（TNFAIP3）和TNFAIP3相互作用蛋白1（TNIP1），在系统性红斑狼疮（SLE）患者中上调。本研究的目的是探讨TNFAIP3/TNIP1β表达是否与SLE发病机制相关，并与TNFAIP3和TNIP1表达相关。采用定量逆转录-聚合酶链反应分析评估20例SLE患者和20例健康对照者外周血单个核细胞（PBMC）中TNFAIP3/TNIP1β、TNFAIP3和TNIP1 mRNA的表达。采用Spearman秩检验确定SLE患者PBMC中TNFAIP3/TNIP1β表达与SLE疾病活动度之间的相关性，以及TNFAIP3/TNIP1β表达与TNFAIP3/TNIP1表达之间的相关性。与健康对照相比，SLE患者TNFAIP3/TNIP1β mRNA表达明显增加。TNFAIP3/TNIP1β的表达与SLE疾病活动指数呈正相关，与补体成分C3和C4的血清水平呈负相关。此外，与抗核、抗史密斯和抗nRNP抗体阳性的SLE患者相比，抗体阴性的SLE患者PBMC中TNIP1β mRNA表达增加。此外，SLE患者中TNFAIP3/TNIP1β的mRNA表达水平与TNFAIP3和TNIP1表达呈正相关。本研究结果表明，PBMC中TNFAIP3/TNIP1β表达增加以及TNFAIP3/TNIP1β与TNFAIP3/TNIP1之间的相互作用可能参与了SLE的发病机制。
