使用标记的[Asu1, 7]-人降钙素类似物进行人降钙素的放射免疫分析。

Radioimmunoassay of human calcitonin using labeled [Asu1, 7]-human calcitonin analogue.

作者信息

Okada Y, Morimoto S, Onishi T, Tanaka K, Tsuji M, Kumahara Y, Tsushima S, Nakazawa N, Ogawa H, Sakakibara S

出版信息

Endocrinol Jpn. 1978 Dec;25(6):617-22. doi: 10.1507/endocrj1954.25.617.

DOI:10.1507/endocrj1954.25.617

Abstract

Application of synthetic human calcitonin analogue ([Asul,7]-hCT) was attempted, since it is chemically more stable than native hCT. [Asu1, 7]-hCT was successfully labeled with 125I to a specific activity ranging from 410 to 540 microCi/microgram. Sensitivity, recovery and reproducibility of the assay system using 125I-labeled [Asu1,7]-hCT analogue, standard synthetic hCT and its antibodies, were 49 pg/ml, 99.8 +/- 4%, and 7.95% (C. V. within assay) or 21.9% (C. V. between assays), respectively. The dilution curve of the sera obtained from patients with medullary carcinoma paralled well with the standard curve. 125-I-labeled [Asu1, 7]-hCT analogue can be used for routine radioimmunoassay of hCT.

摘要

由于合成的人降钙素类似物（[Asu1,7]-hCT）在化学性质上比天然hCT更稳定，因此尝试应用该类似物。[Asu1,7]-hCT成功地用125I标记，比活度范围为410至540微居里/微克。使用125I标记的[Asu1,7]-hCT类似物、标准合成hCT及其抗体的检测系统的灵敏度、回收率和重现性分别为49皮克/毫升、99.8±4%以及7.95%（批内变异系数）或21.9%（批间变异系数）。髓样癌患者血清的稀释曲线与标准曲线吻合良好。125I标记的[Asu1,7]-hCT类似物可用于hCT的常规放射免疫分析。