苏云金芽孢杆菌Cry3Aa和Cry3Ca蛋白在香蕉象甲（Cylas puncticollis，三锥象甲科）中的非共享结合位点
Unshared binding sites for Bacillus thuringiensis Cry3Aa and Cry3Ca proteins in the weevil Cylas puncticollis (Brentidae).
作者信息
Hernández-Martínez Patricia, Vera-Velasco Natalia Mara, Escriche Baltasar
机构信息
Departamento de Genética, Facultad de CC. Biológicas, Universitat de València, Dr Moliner 50, 46100, Burjassot, Spain; Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València, Dr Moliner 50, 46100, Burjassot, Spain.
Departamento de Genética, Facultad de CC. Biológicas, Universitat de València, Dr Moliner 50, 46100, Burjassot, Spain; Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València, Dr Moliner 50, 46100, Burjassot, Spain.
出版信息
Toxicon. 2016 Nov;122:50-53. doi: 10.1016/j.toxicon.2016.09.014. Epub 2016 Sep 20.
Bacillus thuringiensis Cry3Aa and Cry3Ca proteins have been reported to be toxic against the African sweetpotato pest Cylas puncticollis. In the present work, the binding sites of these proteins in C. puncticollis brush border vesicles suggest the occurrence of different binding sites, but only one of them is shared. Our results suggest that pest resistance mediated by alteration of the shared Cry-receptor binding site might not render both Cry proteins ineffective.
据报道，苏云金芽孢杆菌Cry3Aa和Cry3Ca蛋白对非洲甘薯害虫甘薯小象甲有毒性。在本研究中，这些蛋白在甘薯小象甲刷状缘膜泡中的结合位点表明存在不同的结合位点，但只有一个是共享的。我们的结果表明，由共享的Cry受体结合位点改变介导的害虫抗性可能不会使两种Cry蛋白都失效。
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