Perdok J F, van der Mei H C, Genet M J, Rouxhet P G, Busscher H J
Laboratory for Materia Technica, Dental School, Groningen, The Netherlands.
Caries Res. 1989;23(5):297-302. doi: 10.1159/000261196.
In vitro adsorption of chlorhexidine from a commercially available chlorhexidine-containing (0.12%) mouthrinse (Peridex) on both ground and polished and on saliva-coated enamel was studied by X-ray photoelectron spectroscopy and contact angle measurements. Furthermore, adsorption of salivary constituents was studied on chlorhexidine-treated enamel. Changes in the elemental surface composition obtained by X-ray photoelectron spectroscopy clearly demonstrated adsorption of chlorhexidine from the mouthrinse as indicated by increased N and C signals which were in all cases accompanied by a higher enamel surface free energy, estimated from the contact angle data. In addition, it was found that salivary constituents adsorb less to chlorhexidine-treated enamel than to ground and polished enamel and, moreover, that adsorbed salivary constituents were desorbed by chlorhexidine. In vivo, the effect of a 3-week use of chlorhexidine was compared with the use of a placebo and habitual oral hygiene of 10 volunteers by means of clinical contact angle measurements. Also in vivo adsorption of chlorhexidine yielded elevated surface free energies with respect to habitual oral hygiene or a placebo. Thus in vivo, the well-known favourable effects of the bactericidal properties of chlorhexidine completely overrule the unfavourable effects of high enamel surface free energies.
通过X射线光电子能谱和接触角测量，研究了市售含氯己定（0.12%）漱口水（Peridex）中的氯己定在研磨和抛光以及唾液包被的牙釉质上的体外吸附情况。此外，还研究了唾液成分在经氯己定处理的牙釉质上的吸附情况。X射线光电子能谱获得的元素表面组成变化清楚地表明，漱口中的氯己定发生了吸附，这表现为N和C信号增加，在所有情况下，根据接触角数据估计，牙釉质表面自由能也更高。此外，还发现唾液成分在经氯己定处理的牙釉质上的吸附量比在研磨和抛光的牙釉质上少，而且，吸附的唾液成分会被氯己定解吸。在体内，通过临床接触角测量，比较了10名志愿者使用氯己定3周与使用安慰剂及日常口腔卫生习惯的效果。同样在体内，氯己定的吸附也使表面自由能相对于日常口腔卫生习惯或安慰剂有所升高。因此在体内，氯己定杀菌特性的众所周知的有利作用完全超过了牙釉质表面自由能升高的不利影响。
