Rubio C A
J Natl Cancer Inst. 1978 Jul;61(1):259-63. doi: 10.1093/jnci/61.1.259.
The rate of cell division during a 6-hour period was determined in cervical epithelia from 64 normal mice, 26 mice with cervical atypias, and 8 mice with invasive carcinomas. Cervical atypias and carcinomas were induced by local application of benzo[a]pyrene (BP), and mitosis was arrested with colchicine. Values for the ratio of dividing basal cells to total dividing cells and for the rates of basal cell division for the 6-hour period were both significantly greater in normal epithelia than in atypias, and these values for atypias were greater than those for invasive carcinomas. The differences were not estrous cycle-dependent. There was no evidence that the mechanism of host invasion by squamous tumor cells was due to an increased number of cells in mitoses. The occurrence of areas with mitotic activity suggested the existence of focally distributed and probably alternating, synchronous cell production in normal cervical epithelium. This property was apparently not altered by topical BP application or by carcinogenesis.
在6小时期间，测定了64只正常小鼠、26只患有宫颈非典型增生的小鼠和8只患有浸润性癌的小鼠宫颈上皮细胞的分裂速率。通过局部应用苯并[a]芘（BP）诱导宫颈非典型增生和癌，并用秋水仙碱使有丝分裂停止。正常上皮中分裂的基底细胞与总分裂细胞的比率以及6小时期间基底细胞的分裂速率值均显著高于非典型增生，而非典型增生的这些值又高于浸润性癌。这些差异与发情周期无关。没有证据表明鳞状肿瘤细胞侵袭宿主的机制是由于有丝分裂细胞数量增加。有丝分裂活性区域的出现表明正常宫颈上皮中存在局部分布且可能交替、同步的细胞产生。这种特性显然不会因局部应用BP或致癌作用而改变。
