Rubio C A
J Natl Cancer Inst. 1979 Mar;62(3):629-31. doi: 10.1093/jnci/62.3.629.
The autoradiographic patterns of the cervical epithelia of 39 virgin C57BL mice after in vivo injection of [3H]cytidine was investigated. Of the 22 animals painted with benzo[a]pyrene for 5 months, 8 developed intraepithelial atypias and 2 had invasive carcinoma. The mean number of silver grains in individual cells increased from histologically normal cervical epithelium through atypical epithelium to invasive carcinoma. It is apparent that the autoradiographic patterns in normal, atypical, and invasive carcinoma cells reflected quantitative differences in cytidine incorporation. These differences may denote increased demand for RNA precursors in the cervical epithelium during carcinogenesis.
对39只未交配的C57BL小鼠在体内注射[3H]胞苷后，其宫颈上皮的放射自显影模式进行了研究。在22只涂抹苯并[a]芘5个月的动物中，8只出现上皮内异型增生，2只发生浸润性癌。从组织学正常的宫颈上皮到异型上皮再到浸润性癌，单个细胞中银颗粒的平均数量增加。显然，正常、异型和浸润性癌细胞中的放射自显影模式反映了胞苷掺入量的定量差异。这些差异可能表明在致癌过程中宫颈上皮对RNA前体的需求增加。
