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基于激发依赖性荧光的纳米金刚石的植物细胞成像。

Plant Cell Imaging Based on Nanodiamonds with Excitation-Dependent Fluorescence.

机构信息

School of Physics and Engineering, Zhengzhou University, No.75 Daxue Road, Zhengzhou, 450052, China.

出版信息

Nanoscale Res Lett. 2016 Dec;11(1):425. doi: 10.1186/s11671-016-1641-0. Epub 2016 Sep 23.

DOI:10.1186/s11671-016-1641-0
PMID:27664016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5035291/
Abstract

Despite extensive work on fluorescence behavior stemming from color centers of diamond, reports on the excitation-dependent fluorescence of nanodiamonds (NDs) with a large-scale redshift from 400 to 620 nm under different excitation wavelengths are so far much fewer, especially in biological applications. The fluorescence can be attributed to the combined effects of the fraction of sp(2)-hybridized carbon atoms among the surface of the fine diamond nanoparticles and the defect energy trapping states on the surface of the diamond. The excitation-dependent fluorescent NDs have been applied in plant cell imaging for the first time. The results reported in this paper may provide a promising route to multiple-color bioimaging using NDs.

摘要

尽管人们对钻石色心的荧光行为进行了广泛的研究,但迄今为止,关于在不同激发波长下,纳米钻石(NDs)从 400nm 到 620nm 大范围红移的激发依赖性荧光的报道要少得多,特别是在生物应用方面。这种荧光可以归因于精细金刚石纳米粒子表面的 sp(2)-杂化碳原子的分数以及金刚石表面的缺陷能量俘获态的综合影响。激发依赖性荧光 NDs 已首次应用于植物细胞成像。本文报道的结果可能为使用 NDs 进行多色生物成像提供了一条有前途的途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/ab71657e97ea/11671_2016_1641_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/4b8b8ce43d95/11671_2016_1641_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/28d4902586fd/11671_2016_1641_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/41820a3e97aa/11671_2016_1641_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/5bdd5cc83573/11671_2016_1641_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/ab71657e97ea/11671_2016_1641_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/4b8b8ce43d95/11671_2016_1641_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/28d4902586fd/11671_2016_1641_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/41820a3e97aa/11671_2016_1641_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/5bdd5cc83573/11671_2016_1641_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/225f/5035291/ab71657e97ea/11671_2016_1641_Fig5_HTML.jpg

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