School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Nanoscale Res Lett. 2016 Dec;11(1):427. doi: 10.1186/s11671-016-1644-x. Epub 2016 Sep 23.
A nanofluidic biosensor based on nanoreplica molding photonic crystal (PC) was proposed. UV epoxy PC was fabricated by nanoreplica molding on a master PC wafer. The nanochannels were sealed between the gratings on the PC surface and a taped layer. The resonance wavelength of PC-based nanofluidic biosensor was used for testing the sealing effect. According to the peak wavelength value of the sensor, an initial label-free experiment was realized with R6g as the analyte. When the PC-based biosensor was illuminated by a monochromatic light source with a specific angle, the resonance wavelength of the sensor will match with the light source and amplified the electromagnetic field. The amplified electromagnetic field was used to enhance the fluorescence excitation result. The enhancement effect was used for enhancing fluorescence excitation and emission when matched with the resonance condition. Alexa Fluor 635 was used as the target dye excited by 637-nm laser source on a configured photonic crystal enhanced fluorescence (PCEF) setup, and an initial PCEF enhancement factor was obtained.
基于纳米复制模压光子晶体 (PC) 的纳米流体生物传感器被提出。通过在母版 PC 晶圆上进行纳米复制模压，制造出了 UV 环氧树脂 PC。纳米通道被密封在 PC 表面的光栅和胶带层之间。基于 PC 的纳米流体生物传感器的共振波长用于测试密封效果。根据传感器的峰值波长值，使用 R6g 作为分析物进行了初始的无标记实验。当基于 PC 的生物传感器被特定角度的单色光源照射时，传感器的共振波长将与光源匹配并放大电磁场。放大的电磁场用于增强荧光激发结果。当与共振条件匹配时，增强效果用于增强荧光激发和发射。Alexa Fluor 635 被用作配置的光子晶体增强荧光 (PCEF) 装置上由 637nm 激光源激发的目标染料，并获得了初始的 PCEF 增强因子。
