Liu Lei, Zhu Lizhong
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanics, Southeast University, Si Pai Lou 2#, Nanjing, 210096 People's Republic of China ; Suzhou Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Suzhou Research Institute of Southeast University, Linquan street 399#, Suzhou, 215123 People's Republic of China.
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanics, Southeast University, Si Pai Lou 2#, Nanjing, 210096 People's Republic of China.
Nanoscale Res Lett. 2015 Apr 25;10:198. doi: 10.1186/s11671-015-0889-0. eCollection 2015.
In this work, a nanofluidic device containing both a feed cell and a permeation cell linked by nanopore arrays has been fabricated, which is employed to investigate thermally controlled biomolecular transporting properties through confined nanochannels. The ionic currents modulated by the translocations of goat antibody to human immunoglobulin G (IgG) or bovine serum albumin (BSA) are recorded and analyzed. The results suggest that the modulation effect decreases with the electrolyte concentration increasing, while the effects generated by IgG translocation are more significant than that generated by BSA translocation. More importantly, there is a maximum decreasing value in each modulated current curve with biomolecule concentration increasing for thermally induced intermolecular collision. Furthermore, the turning point for the maximum shifts to lower biomolecule concentrations with the system temperature rising (from 4°C to 45°C), and it is mainly determined by the temperature in the feed cell if the temperature difference exists in the two separated cells. These findings are expected to be valuable for the future design of novel sensing device based on nanopore and/or nanopore arrays.
在这项工作中，制造了一种纳米流体装置，该装置包含通过纳米孔阵列连接的进料池和渗透池，用于研究通过受限纳米通道的热控生物分子传输特性。记录并分析了由山羊抗体与人免疫球蛋白G（IgG）或牛血清白蛋白（BSA）的易位调制的离子电流。结果表明，调制效果随电解质浓度的增加而降低，而IgG易位产生的效果比BSA易位产生的效果更显著。更重要的是，对于热诱导的分子间碰撞，随着生物分子浓度的增加，每条调制电流曲线都有一个最大下降值。此外，随着系统温度升高（从4°C到45°C），最大值的转折点向较低的生物分子浓度移动，如果两个分离的池中存在温差，则主要由进料池中的温度决定。这些发现有望对基于纳米孔和/或纳米孔阵列的新型传感装置的未来设计具有重要价值。
